ZO-2 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

ZO-2 Rekombinant Antikörper für WB, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra)

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230474D1

Kat-Nr. : CL750-83111

Synonyme

TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2

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  WB analysis using CL750-83111

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83111 (ZO-2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of A431 using CL750-83111

  1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated ZO-2 Recombinant antibody (CL750-83111, Clone:230474D1)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL750-83111 bindet in WB, FC (Intra) ZO-2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZO-2 fusion protein Ag13534
• Vollständiger Name: tight junction protein 2 (zona occludens 2)
• Berechnetes Molekulargewicht: 134 kDa
• Beobachtetes Molekulargewicht: ~150 kDa
• GenBank-Zugangsnummer: BC027592
• Gene symbol: TJP2
• Gene ID (NCBI): 9414
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.