acetylated Tubulin(Lys40) Monoklonaler Antikörper

acetylated Tubulin(Lys40) Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Hund, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen

3

CloneNo.

7E5H8

Kat-Nr. : CL488-66200

Synonyme

acetylated Tubulin (Lys40), acetylated Tubulin(Lys40), acetyl-tubulin

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Galerie der Validierungsdaten

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  IF Staining of MDCK using CL488-66200

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CL488-66200 (GREEN, acetylated Tubulin(Lys40) antibody) at dilution of 1:50 and CL594-17711 (RED, ARL13B antibody) at dilution of 1:50.

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  IF Staining of mouse kidney using CL488-66200

  Frozen tissue section of adult mouse kidney was stained for acetylated ɑ-tubulin (magenta, Cat. No CL488-66200), CD-31/PECAM-1 (white), and Shh (green, Cat. No 20697-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). acetylated ɑ-tubulin stains primary cilia and was conjugated to Coralite-488 fluorescent dye and psedocolored to magenta. CD-31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Shh stains Sonic Hedgehog and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to green.The Image and figure legends are intellectual property of @LAF_in_the_LAB.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: MDCK-Zellen, Mausnierengewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 3 publications below

Produktinformation

CL488-66200 bindet in IF acetylated Tubulin(Lys40) und zeigt Reaktivität mit Hausschwein, human, Hund, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tubulin, alpha 1a
• Berechnetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: NM_006009
• Gene symbol: TUBA1A
• Gene ID (NCBI): 7846
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin; it does not recognize non-acetylated tubulin. (24268707, 23881549). This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publikationen

Species	Application	Title
rat	IF	Front Cell Dev Biol; PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia; Authors - Tingting Zhang
human	IF	STAR Protoc; Isolation, expansion, differentiation, and histological processing of human nasal epithelial cells; Authors - Vincent Manna
human	IF	BBA Adv; The dynamic nature of the coronavirus receptor, angiotensin-converting enzyme 2 (ACE2) in differentiating airway epithelia.; Authors - Vincent J Manna