• Featured Product
  • KD/KO Validated

Alpha Tubulin Monoklonaler Antikörper

Alpha Tubulin Monoklonal Antikörper für WB, IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(5)

CloneNo.

1E4C11

Kat-Nr. : CL488-66031

Synonyme

TUBA1B, Tubulin alpha 1B chain, Tubulin alpha, EC:3.6.5.-, Alpha-tubulin ubiquitous



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IF/ICCHeLa-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:300-1:600
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 4 publications below

Produktinformation

CL488-66031 bindet in WB, IF/ICC, FC (Intra) Alpha Tubulin und zeigt Reaktivität mit human, Hund, Maus, Ratten

Getestete Reaktivität human, Hund, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Alpha Tubulin fusion protein Ag18034
Vollständiger Name tubulin, alpha 1b
Berechnetes Molekulargewicht 50 kDa
Beobachtetes Molekulargewicht50-55 kDa
GenBank-ZugangsnummerBC009314
Gene symbol Alpha Tubulin
Gene ID (NCBI) 10376
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CL Plus 488 Alpha Tubulin antibody CL488-66031Protokoll herunterladen
IF protocol for CL Plus 488 Alpha Tubulin antibody CL488-66031Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
IF

Free Radic Biol Med

Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling

Authors - Yaoqiu Wu

Clin Genet

A novel homozygous CFAP65 mutation in humans causes male infertility with multiple morphological abnormalities of the sperm flagella.

Authors - Xueguang Zhang
humanIF

Transl Androl Urol

Novel bi-allelic mutations in DNAH1 cause multiple morphological abnormalities of the sperm flagella resulting in male infertility.

Authors - Chuan Jiang
mouseIF

Reprod Toxicol

Bisphenol M inhibits mouse oocyte maturation in vitro by disrupting cytoskeleton architecture and cell cycle processes

Authors - Huilei Chen
mouseIF

Am J Physiol Cell Physiol

Melatonin partially rescues defects induced by tranexamic acid exposure during oocyte maturation in mice

Authors - Fei Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Pierre (Verified Customer) (09-24-2025)

Worked well

  • Applications: Immunofluorescence
  • Cell Tissue Type: T cells