Beta Actin Monoklonaler Antikörper

Beta Actin Monoklonal Antikörper für WB, IF/ICC

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human, Hund, Maus, Pflanze, Ratte, Zebrafisch

Anwendung

WB, IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(4)

CloneNo.

7D2C10

Kat-Nr. : CL594-60008

Synonyme

ACTB, Actin, bate actin, β actin, Actin cytoplasmic 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, HEK-293-Zellen, HeLa-Zellen
Erfolgreiche Detektion in IF/ICCHeLa-Zellen, MDCK-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 3 publications below
IFSee 1 publications below

Produktinformation

CL594-60008 bindet in WB, IF/ICC Beta Actin und zeigt Reaktivität mit human, Hund, Maus, Pflanze, Ratten, Zebrafisch

Getestete Reaktivität human, Hund, Maus, Pflanze, Ratte, Zebrafisch
In Publikationen genannte ReaktivitätRatte
Wirt / Isotyp Maus / IgM
Klonalität Monoklonal
Typ Antikörper
Immunogen Beta Actin fusion protein Ag0297
Vollständiger Name actin, beta
Berechnetes Molekulargewicht 375 aa, 42 kDa
Beobachtetes Molekulargewicht 42 kDa
GenBank-ZugangsnummerBC002409
Gene symbol Beta Actin
Gene ID (NCBI) 60
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Caprylsäure/Ammoniumsulfat-Präzipitation
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody is conjugated with CL594,Ex/Em 593 nm/614 nm.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CL594 Beta Actin antibody CL594-60008Protokoll herunterladen
IF protocol for CL594 Beta Actin antibody CL594-60008Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
WB

Sci Rep

Translational enhancement of target endogenous mRNA in mammalian cells using programmable RNA-binding pentatricopeptide repeat proteins

Authors - Ning Ping
ratWB

Neurosci Res

Hippocampal Warburg effect Mediates Hydrogen Sulfide-Ameliorated Diabetes-Associated Cognitive Dysfunction: Involving Promotion of Hippocampal Synaptic Plasticity

Authors - Run-Qi Li
IF

Proc Natl Acad Sci U S A

Multifunctional biomolecular corona-inspired nanoremediation of antibiotic residues

Authors - Jie Hou
WB

Aging Cell

CaMKIIα-TARPγ8 signaling mediates hippocampal synaptic impairment in aging

Authors - Zhao JianHua