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cyclin B1 Monoklonaler Antikörper

cyclin B1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(26)

CloneNo.

1F3G11

Kat-Nr. : 67686-1-Ig

Synonyme

CCNB1, 1F3G11, CCNB, Cyclin B, G2/mitotic specific cyclin B1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IHChumanes Tonsillitisgewebe, Rattenhautgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen, HT-29-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

67686-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA cyclin B1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen cyclin B1 fusion protein Ag29444
Vollständiger Name cyclin B1
Berechnetes Molekulargewicht 48 kDa
Beobachtetes Molekulargewicht 55 kDa
GenBank-ZugangsnummerBC006510
Gene symbol Cyclin B1
Gene ID (NCBI) 891
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase of the cell cycle. The different transcripts result from the use of alternate transcription initiation sites. The antibody is specific to CCNB1. We got a 55-60 kDa band in western blotting maybe due to phosphorylation.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for cyclin B1 antibody 67686-1-IgProtokoll herunterladen
IHC protocol for cyclin B1 antibody 67686-1-IgProtokoll herunterladenl
IF protocol for cyclin B1 antibody 67686-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer

Authors - Rong Wang
humanWB

Ecotoxicol Environ Saf

The role of ER stress and ATP/AMPK in oxidative stress meditated hepatotoxicity induced by citrinin.

Authors - Jing Wu
mouse,humanWB,IF

Cell Signal

MicroRNA-30c-5p arrests bladder cancer G2/M phase and suppresses its progression by targeting PRC1-mediated blocking of CDK1/Cyclin B1 axis

Authors - Yining Hao
humanWB

Front Mol Biosci

Over-Expression and Prognostic Significance of FATP5, as a New Biomarker, in Colorectal Carcinoma.

Authors - Qi-Shun Geng
humanWB

Steroids

The location of estrogen receptor variant ER-α36 is associated with the invasion of glioblastoma

Authors - Hongyan Li
humanWB

Cell Biochem Biophys

Brucine Suppresses Malignant Progression of Prostate Cancer by Decreasing Sarcosine Accumulation via Downregulation of GNMT in the Glycine/sarcosine Metabolic Pathway

Authors - Long Miao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Tsimafei (Verified Customer) (08-04-2024)

Multiple bands are detected on the membrane

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mESCs
cyclin B1 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:67686-1-Ig)