Lamin A/C Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Lamin A/C Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

183

Rezensionen (5)

Kat-Nr. : 10298-1-AP

Synonyme

70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1, progerin

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• Galerie der Validierungsdaten
• Proteintech vs. Other Vendors

• 

  WB analysis using 10298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C antibody) at dilution of 1:35000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10298-1-AP

  WB result of Lamin A/C antibody (10298-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin A/C transfected HeLa cells.

• 

  WB analysis of C6 using 10298-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10298-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 10298-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 10298-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10298-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10298-1-AP

  IP result of anti-Lamin A/C(IP:10298-1-AP, 4ug; Detection:10298-1-AP 1:50000) with HeLa cells lysate 1360 ug.

• 

  IP experiment of A375 using 10298-1-AP

  IP Result of anti-lamin-A (IP:10298-1-AP, 3ug; Detection:10298-1-AP 1:1000) with A375 cells lysate 800ug.

• 

  IHC staining of mouse heart using 10298-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10298-1-AP (Lamin A/C antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 10298-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10298-1-AP (Lamin A/C antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10298-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10298-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin A/C antibody (10298-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 10298-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10298-1-AP

  Immunofluorescent analysis of (-20°C Acetone) fixed HeLa cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:100 and CL594-66467 (CL594-Mouse anti-Rabbit IgG heavy chain) as secondary antibody with dilution 1:400. .

• 

  FC experiment of HEK-293T using 10298-1-AP

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human Lamin A/C (10298-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A375-Zellen, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, HUVEC-Zellen, Maus-Eierstockgewebe, NIH/3T3-Zellen, SKOV-3-Zellen
• Erfolgreiche IP: HeLa-Zellen, A375-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC: HEK-293T-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF): IF : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 172 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• IP: See 3 publications below

Produktinformation

10298-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Lamin A/C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Ente, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Lamin A/C fusion protein Ag0408
• Vollständiger Name: lamin A/C
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa, 70 kDa
• GenBank-Zugangsnummer: BC003162
• Gene symbol: LMNA
• Gene ID (NCBI): 4000
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lamin A/C is also named as LMNA or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure, and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb-girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa, and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.

Publikationen

Species	Application	Title
human	IF	Nat Microbiol; Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus.; Authors - Adarsh Dharan
human	WB	Mol Cell; Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells.; Authors - Jia Xiong
human	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
human	WB	Cancer Cell; SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis.; Authors - Lan Fang
human	WB	Sci Adv; XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility; Authors - Ming Kuang
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alejandro (Verified Customer) (08-22-2022)

Shows clear staining in IF and well staining using FACS

• Applications: Immunofluorescence, Flow Cytometry
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Bone marrow derived macrophages

Lamin A/C Antibody Immunofluorescence,Flow Cytometry validation (1/100 dilution) in Bone marrow derived macrophages (Cat no:10298-1-AP)

FH

Charlotte (Verified Customer) (07-29-2022)

Cell fraction performed on NIH-3T3 cells to show the nucleus part. Blot super clean. Antibody specific. Easy to reveal.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: NIH-3T3

Lamin A/C Antibody Western Blot validation (1/1000 dilution) in NIH-3T3 (Cat no:10298-1-AP)

FH

S (Verified Customer) (12-31-2021)

good antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

Lamin A/C Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:10298-1-AP)

FH

Azita (Verified Customer) (06-16-2021)

It was used as a marker in WB to confirm proper nuclear fractionation of cell lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NSC-34 cell line

FH

Declan (Verified Customer) (11-29-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: Human brain homogenate

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