mCherry Polyklonaler Antikörper

mCherry Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein und mehr (5)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

57

Rezensionen (6)

Kat-Nr. : 26765-1-AP

Synonyme

mCherry

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Galerie der Validierungsdaten

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  WB analysis of Transfected HEK-293 using 26765-1-AP

  WB result of mCherry antibody (26765-1-AP; 1:2000; room temperature for 1.5 hours) with negative control and mCherry overexpressed Transfected HEK-293 cells.

• 

  WB analysis of Transfected HEK-293 using 26765-1-AP

  WB result of mCherry antibody (26765-1-AP; 1:300; room temperature for 1.5 hours) with negative control and mCherry overexpressed Transfected HEK-293 cells.

• 

  IF Staining of Transfected HEK-293 using 26765-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using mCherry antibody (26765-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IF: Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 37 publications below
• IHC: See 2 publications below
• IF: See 21 publications below
• IP: See 6 publications below
• CoIP: See 3 publications below

Produktinformation

26765-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA mCherry und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: human, Hefe, Maus, Pflanze, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: mCherry fusion protein Ag25320
• Vollständiger Name: mCherry
• Berechnetes Molekulargewicht: 27 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively.

Publikationen

Species	Application	Title
human	IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu
human	IP	Nat Commun; DELE1 tracks perturbed protein import and processing in human mitochondria.; Authors - Evelyn Fessler
human	IP	Protein Cell; cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence.; Authors - Xiaocui Li
human	IF,IP	Dev Cell; ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress; Authors - Di Chen
	IF	Dev Cell; Centrosomal Localization of RXRα Promotes PLK1 Activation and Mitotic Progression and Constitutes a Tumor Vulnerability.; Authors - Guobin Xie
	WB,IF	Autophagy; Proteotoxic stress disrupts epithelial integrity by inducing MTOR sequestration and autophagy overactivation.; Authors - Xiaoxiang Cheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Verdiana (Verified Customer) (10-12-2023)

Nice and clear bands!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS

FH

Andrea (Verified Customer) (10-05-2023)

Good and strong signal for the mCherry fusion protein. In my case it was a 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT-116

mCherry Antibody Western Blot validation (1:1000 dilution) in HCT-116 (Cat no:26765-1-AP)

FH

Tatyana (Verified Customer) (01-09-2023)

Excellent antibody, very good bright signal, no background or non-specific bands. HeLa cells were transfected with plasmids for expression of mCherry tagged proteins, lysed after 24 hours, followed by WB (semi-dry transfer, blocking in 4% milk). Antibody was diluted in 4% milk.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human / HeLa cells

mCherry Antibody Western Blot validation (1:2000 dilution) in Human / HeLa cells (Cat no:26765-1-AP)

FH

Laura (Verified Customer) (01-14-2020)

Antibody was used in 5% TBST overnight, gave a clean blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HEK293T

FH

Benjamin (Verified Customer) (12-10-2019)

This antibody has been used to image mCherry fusion proteins expressed in HEK293T cells via western blot with high rates of success.Antibody was diluted in 3% milk TBST and incubated overnight at 4 degrees.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HEK293T Lysate

FH

Liang (Verified Customer) (05-27-2019)

The antibody works perfect. According the attached image, The 2nd from the left is the negative control protein (mGFP) and the 3rd lane is the mCherry protein as the positive control, the others are the target protein.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: purified protein expressed from Ecoli

mCherry Antibody Western Blot,Immunoprecipitation, validation (1:3000 dilution) in purified protein expressed from Ecoli (Cat no:26765-1-AP)