CISD1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CISD1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

38

Kat-Nr. : 16006-1-AP

Synonyme

C10orf70, CDGSH iron sulfur domain 1, CISD1, MDS029, mitoNEET, Mnt, ZCD1

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Galerie der Validierungsdaten

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  WB analysis using 16006-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16006-1-AP (CISD1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16006-1-AP

  IP Result of anti-CISD1 (IP:16006-1-AP, 3ug; Detection:16006-1-AP 1:2000) with HepG2 cells lysate 600ug.

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  IHC staining of human pancreas cancer using 16006-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16006-1-AP (CISD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16006-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CISD1 antibody (16006-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 16006-1-AP

  1X10^6 HeLa cells were stained with .05ug mitoNEET,CISD1 antibody (16006-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-Goat anti-Rabbit IgG with dilution 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Maus-Skelettmuskelgewebe, Rattennierengewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.05 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 33 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

16006-1-AP bindet in WB, IP, IHC, IF, FC, ELISA CISD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CISD1 fusion protein Ag8680
• Vollständiger Name: CDGSH iron sulfur domain 1
• Berechnetes Molekulargewicht: 108 aa, 12 kDa
• Beobachtetes Molekulargewicht: 14-17 kDa
• GenBank-Zugangsnummer: BC007043
• Gene symbol: CISD1
• Gene ID (NCBI): 55847
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.

Publikationen

Species	Application	Title
human	WB	Nat Struct Mol Biol; Mechanism and regulation of the Lys6-selective deubiquitinase USP30.; Authors - Malte Gersch
mouse	WB	Cell Metab; Basal Mitophagy Occurs Independently of PINK1 in Mouse Tissues of High Metabolic Demand.; Authors - Thomas G McWilliams
human	WB	Mol Cell; Quantitative proteomics reveal a feedforward mechanism for mitochondrial PARKIN translocation and ubiquitin chain synthesis.; Authors - Alban Ordureau
human	WB	Mol Cell; Dynamics of PARKIN-Dependent Mitochondrial Ubiquitylation in Induced Neurons and Model Systems Revealed by Digital Snapshot Proteomics.; Authors - Alban Ordureau
		Mol Cell; Global Landscape and Dynamics of Parkin and USP30-Dependent Ubiquitylomes in iNeurons during Mitophagic Signaling.; Authors - Alban Ordureau
human	WB	Sci Adv; AMPK/ULK1-mediated phosphorylation of Parkin ACT domain mediates an early step in mitophagy.; Authors - Chien-Min Hung

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