p70(S6K) Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

p70(S6K) Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

144

Kat-Nr. : 14485-1-AP

Synonyme

p70 alpha, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K 1, p70 S6K alpha, p70 S6KA, p70(S6K), p70(S6K) alpha, p70S6K, P70S6K1, PS6K, Ribosomal protein S6 kinase I, RPS6KB1, S6K, S6K beta 1, S6K1, STK14A

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 14485-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70(S6K) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14485-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70(S6K) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14485-1-AP

  WB result of p70(S6K) antibody (14485-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p70(S6K) transfected MCF-7 cells.

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  WB analysis using 14485-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14485-1-AP (p70(S6K) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14485-1-AP

  IP Result of anti-p70S6K (IP:14485-1-AP, 3ug; Detection:14485-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of human liver cancer using 14485-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14485-1-AP (p70(S6K) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14485-1-AP

  Immunofluorescent analysis of HepG2 cells using 14485-1-AP (p70S6K antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HepG2 using 14485-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p70(S6K) antibody (14485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 14485-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug p70S6K antibody (14485-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C6-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, Mit IGF-1 behandelte MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 140 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

14485-1-AP bindet in WB, IP, IHC, IF, FC, ELISA p70(S6K) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Kaninchen, Maus, Ratte, Riesenscheibenbrasse (Megalobrama amblycephala)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: p70(S6K) fusion protein Ag5883
• Vollständiger Name: ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 65-75 kDa, 80-85 kDa
• GenBank-Zugangsnummer: BC053365
• Gene symbol: RPS6KB1
• Gene ID (NCBI): 6198
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA, and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of mTOR and acts as a crucial effector of mTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K). The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation, and metabolism and is the central regulator of translation of some components of the protein synthesis system. Due to alternative translation, two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1).

Publikationen

Species	Application	Title
human,mouse	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
mouse	WB	Cell Stem Cell; Amino acid catabolism regulates hematopoietic stem cell proteostasis via a GCN2-eIF2α axis.; Authors - Changzheng Li
mouse	IF	Small Methods; PROTAC Degraders of Androgen Receptor-Integrated Dissolving Microneedles for Androgenetic Alopecia and Recrudescence Treatment via Single Topical Administration; Authors - Ruxuan Wang
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human,mouse	WB	Redox Biol; Aberrant hyper-expression of the RNA binding protein GIGYF2 in endothelial cells modulates vascular aging and function; Authors - Fanglin Niu
mouse	WB	Autophagy; SMCR8 negatively regulates AKT and MTORC1 signaling to modulate lysosome biogenesis and tissue homeostasis.; Authors - Yungang Lan

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