Alpha smooth muscle actin specific Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Alpha smooth muscle actin specific Rekombinant Antikörper für IF/ICC, IF-P, IF-Fro, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, Huhn, human, Maus, Ratte

Anwendung

WB, IF/ICC, IF-P, IF-Fro, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Publikationen(1)

CloneNo.

5H7

Kat-Nr. : CL594-80008

Synonyme

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, 5H7

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  IF Staining of mouse heart using CL594-80008

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7 ) at dilution of 1:200.

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  IF Staining of mouse small intestine using CL594-80008

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using CoraLite®594 Alpha smooth muscle actin specific antibody (CL594-80008, Clone: 5H7 ) at dilution of 1:200.

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  IF Staining of H9C2 using CL594-80008

  Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7 ) at dilution of 1:200.

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  FC experiment of C2C12 using CL594-80008

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human smooth muscle actin specific (CL594-80008, Clone:5H7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Mausherzgewebe
• Erfolgreiche Detektion in IF-Fro: Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IF/ICC: H9C2-Zellen
• Erfolgreiche Detektion in FC (Intra): C2C12-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

CL594-80008 bindet in WB, IF/ICC, IF-P, IF-Fro, FC (Intra) Alpha smooth muscle actin specific und zeigt Reaktivität mit Hausschwein, Huhn, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: actin, alpha 2, smooth muscle, aorta
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: NM_001613
• Gene symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Publikationen

Species	Application	Title
human	WB	Toxicol Appl Pharmacol; Mechanistic role for mTORC1 signaling in profibrotic toxicity of low-dose cadmium; Authors - Choon-Myung Lee