Alpha smooth muscle actin specific Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Alpha smooth muscle actin specific Rekombinant Antikörper für IF-P, IF-Fro

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, Huhn, human, Maus, Ratte, Zebrafisch

Anwendung

IF-P, IF-Fro

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

5H7

Kat-Nr. : CL647-80008

Synonyme

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, 5H7

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  IF Staining of mouse small intestine using CL647-80008

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7 ) at dilution of 1:200, ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), ASCT2 antibody (20350-1-AP, green).

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  IF Staining of mouse small intestine using CL647-80008

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7 ) at dilution of 1:200, ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), ASCT2 antibody (20350-1-AP, green).

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  IF Staining of mouse small intestine using CL647-80008

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using CoraLite® Plus 647 Alpha smooth muscle actin specific antibody (CL647-80008, Clone: 5H7 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IF-Fro: Maus-Dünndarmgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-80008 bindet in IF-P, IF-Fro Alpha smooth muscle actin specific und zeigt Reaktivität mit Hausschwein, Huhn, human, Maus, Ratten, Zebrafisch

• Getestete Reaktivität: Hausschwein, Huhn, human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: actin, alpha 2, smooth muscle, aorta
• Berechnetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: NM_001613
• Gene symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.