Gamma Tubulin Polyklonaler Antikörper

Gamma Tubulin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26195-1-AP

Synonyme

TUBG1, Tubulin gamma 1 chain, TUBG, GCP-1, Gamma-tubulin complex component 1

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  WB analysis of mouse brain using 26195-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26195-1-AP (gamma tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26195-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26195-1-AP (gamma tubulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 26195-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 26195-1-AP (Gamma Tubulin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 26195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Gamma Tubulin antibody (26195-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of C2C12 using 26195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Gamma Tubulin antibody (26195-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of MDCK using 26195-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26195-1-AP (gamma tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MDCK using 26195-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26195-1-AP (gamma tubulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 26195-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 26195-1-AP (gamma tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26195-1-AP bindet in WB, IHC, IF/ICC, ELISA Gamma Tubulin und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Gamma Tubulin fusion protein Ag23575
• Vollständiger Name: tubulin, gamma 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC000619
• Gene symbol: Gamma Tubulin
• Gene ID (NCBI): 7283
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.