Beta ENaC Polyklonaler Antikörper

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• KD/KO Validated

Beta ENaC Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 14134-1-AP

Synonyme

Beta ENaC, Beta NaCH, ENaCb, ENaCbeta, SCNEB, SCNN1B, β ENaC, βENaC

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 14134-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14134-1-AP (β-ENaC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 14134-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14134-1-AP (β-ENaC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14134-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14134-1-AP (β-ENaC Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 14134-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14134-1-AP (β-ENaC Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below

Produktinformation

14134-1-AP bindet in WB, IHC, ELISA Beta ENaC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Beta ENaC fusion protein Ag5348
• Vollständiger Name: sodium channel, nonvoltage-gated 1, beta
• Berechnetes Molekulargewicht: 640 aa, 73 kDa
• Beobachtetes Molekulargewicht: 75-80 kDa
• GenBank-Zugangsnummer: BC036352
• Gene symbol: SCNN1B
• Gene ID (NCBI): 6338
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCNN1B, also named as SCNEB, ENaCB, Beta-NaCH and Beta-ENaC, belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family and SCNN1B subfamily. Sodium permeable non-voltage-sensitive ion channel is inhibited by the diuretic amiloride. SCNN1B mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. It controls the reabsorption of sodium in kidney, colon, lung and sweat glands. SCNN1B plays a role in taste perception.

Publikationen

Species	Application	Title
human	IHC	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
pig	WB	Anim Nutr; Glutamine in suppression of lipopolysaccharide-induced piglet intestinal inflammation: The crosstalk between AMPK activation and mitochondrial function.; Authors - Liuqin He
mouse	WB	Front Pharmacol; Melatonin Attenuates Sepsis-Induced Acute Lung Injury Through Improvement of Epithelial Sodium Channel-Mediated Alveolar Fluid Clearance Via Activation of SIRT1/SGK1/Nedd4-2 Signaling Pathway.; Authors - Jing Li
rat	WB	J Neurotrauma; Activity-based training reverses SCI-induced changes in kidney receptor densities and membrane proteins.; Authors - Jason H Gumbel
hamster	WB	Exp Cell Res; Calreticulin positively regulates the expression and function of epithelial sodium channel.; Authors - Sugahara Takuya T ; KD Validated
human	WB	Toxins (Basel); Listeriolysin O Causes ENaC Dysfunction in Human Airway Epithelial Cells.; Authors - Guang Yang

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