Anticorps Polyclonal de lapin anti-PPP3CC-specific

PPP3CC-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 55163-1-AP

Synonymes

PPP3CC, CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3

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Galerie de données de validation

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  WB analysis of fetal human brain using 55163-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 55163-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55163-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55163-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55163-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 55163-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 55163-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, tissu cérébral humain, tissu testiculaire humain
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

55163-1-AP cible PPP3CC-specific dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: NM_005605
• Symbole du gène: PPP3CC
• Identification du gène (NCBI): 5533
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP3CC, also named as CALNA3 and CNA3, belongs to the PPP phosphatase family and PP-2B subfamily. It is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin. This antibody is specific to PPP3CC.

Publications

Species	Application	Title
human	WB	Acta Biomater; Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.; Authors - Hong Cao
rat	IHC	BMC Pregnancy Childbirth; Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy; Authors - Yan Fang

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