Anticorps Recombinant de lapin anti-6*His, His-Tag

6*His, His-Tag Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

n/a

Applications

FC (Intra)

Conjugaison

APC Fluorescent Dye

CloneNo.

251107A1

N° de cat : APC-FcA98450

Synonymes

6*His, His Tag, His Tag, 251107A1, 6*His, 6*his tag

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Galerie de données de validation

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  FC experiment of Transfected HEK-293 using APC-FcA98450

  1x10^6 HEK-293T or transfected HEK-293T cells were intracellularly stained with 0.1 ug APC Anti-6*His, His-Tag Rabbit RecAb (APC-FcA98450, Clone: 251107A1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Transfected HEK-293 using APC-FcA98450

  1x10^6 transfected HEK-293T cells were intracellularly stained with 0.1 ug APC Anti-6*His, His-Tag Rabbit RecAb (APC-FcA98450, Clone: 251107A1) (red) or 0.1 ug APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9) (blue). 1x10^6 untransfected HEK-293T cells were intracellularly stained with 0.1 ug APC Anti-6*His, His-Tag Rabbit RecAb (APC-FcA98450, Clone: 251107A1) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): cellules HEK-293 transfectées,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-FcA98450 cible 6*His, His-Tag dans les applications de FC (Intra) et montre une réactivité avec des échantillons n/a

• Réactivité: n/a
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: unknow
• Nom complet: 6*His, His-Tag
• Masse moléculaire calculée: 1 kDa
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

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