Anticorps Recombinant de lapin anti-6*His, His-Tag

6*His, His-Tag Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante

Applications

FC (Intra)

Conjugaison

Biotin

CloneNo.

251107A1

N° de cat : Biotin-FcA98450

Synonymes

6*His, His Tag, His Tag, 6*His, 6*his tag, 6*his,His tag

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using Biotin-FcA98450

  1x10^6 untransfected HEK-293T cells or transfected HEK-293T cells were intracellularly stained with 0.25 ug Biotin Anti-His tag Rabbit RecAb (Biotin-FcA98450, Clone: 251107A1) and APC-conjugated streptavidin. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): Transfected HEK-293T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-FcA98450 cible 6*His, His-Tag dans les applications de FC (Intra) et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: unknow
• Nom complet: 6*His, His-Tag
• Masse moléculaire calculée: 1 kDa
• Identification du gène (NCBI): 5
• Conjugaison: Biotin
• Excitation/Emission maxima wavelengths: -
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

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