Anticorps Polyclonal de lapin anti-Alpha 1B-Glycoprotein

Alpha 1B-Glycoprotein Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 14181-1-AP

Synonymes

A1BG, A1B, Alpha-1-B glycoprotein, Alpha 1B glycoprotein, alpha 1 B glycoprotein

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Galerie de données de validation

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  WB analysis of human plasma using 14181-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 14181-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14181-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14181-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 14181-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 14181-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14181-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:25 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain, cellules BxPC-3, cellules MCF-7, tissu rénal de souris
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

14181-1-AP cible Alpha 1B-Glycoprotein dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha 1B-Glycoprotein Protéine recombinante Ag5414
• Nom complet: alpha-1-B glycoprotein
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC035719
• Symbole du gène: A1BG
• Identification du gène (NCBI): 1
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).

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