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Anticorps Polyclonal de lapin anti-Alpha 1B-Glycoprotein

Alpha 1B-Glycoprotein Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 14181-1-AP

Synonymes

A1B, A1BG, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein, Alpha 1B-Glycoprotein, GAB, HYST2477

Galerie de données de validation

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  • Western Blot (WB) analysis of human plasma using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    WB analysis of human plasma using 14181-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    WB analysis of BxPC-3 using 14181-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    WB analysis of MCF-7 using 14181-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    WB analysis of mouse kidney using 14181-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    IHC staining of human hepatocirrhosis using 14181-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    IHC staining of human hepatocirrhosis using 14181-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    IF Staining of HepG2 using 14181-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:25 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Applications testées

Résultats positifs en WBtissu plasmatique humain, cellules BxPC-3, cellules MCF-7, tissu rénal de souris
Résultats positifs en IHCtissu de cirrhose hépatique humain,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IFcellules HepG2

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochimie (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

14181-1-AP cible Alpha 1B-Glycoprotein dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Alpha 1B-Glycoprotein Protéine recombinante Ag5414
Nom complet alpha-1-B glycoprotein
Masse moléculaire calculée 54 kDa
Poids moléculaire observé 65 kDa
Numéro d’acquisition GenBankBC035719
Symbole du gène A1BG
Identification du gène (NCBI) 1
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).

Protocole

Product Specific Protocols
WB protocol for Alpha 1B-Glycoprotein antibody 14181-1-APDownload protocol
IHC protocol for Alpha 1B-Glycoprotein antibody 14181-1-APDownload protocol
IF protocol for Alpha 1B-Glycoprotein antibody 14181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols