Anticorps Polyclonal de lapin anti-Alpha-2-Macroglobulin

Alpha-2-Macroglobulin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 13545-1-AP

Synonymes

A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M

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Galerie de données de validation

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  WB analysis of human plasma using 13545-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13545-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13545-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13545-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13545-1-AP

  IP result of anti-Alpha-2-Macroglobulin(IP:13545-1-AP, 4ug; Detection:13545-1-AP 1:2000) with HepG2 cells lysate 1080 ug.

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  IHC staining of human liver using 13545-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 13545-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13545-1-AP (Alpha-2-macroglobulin antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 13545-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (13545-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu sanguin humain, cellules HepG2, tissu cérébral humain, tissu plasmatique humain
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

13545-1-AP cible Alpha-2-Macroglobulin dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, Plasmodium falciparum
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha-2-Macroglobulin Protéine recombinante Ag4489
• Nom complet: alpha-2-macroglobulin
• Masse moléculaire calculée: 1474 aa, 163 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC040071
• Symbole du gène: A2M
• Identification du gène (NCBI): 2
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Biometal Dyshomeostasis in Olfactory Mucosa of Alzheimer's Disease Patients.; Authors - Riikka Lampinen
Plasmodium falciparum	WB	Malar J; The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.; Authors - Takahiro Tougan