Anticorps Polyclonal de lapin anti-ABCF1

• Phare
• Validé par KD/KO

ABCF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 13950-1-AP

Synonymes

TNF-alpha-stimulated ABC protein, ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, ATP binding cassette 50, ABCF 1

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Galerie de données de validation

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  WB analysis using 13950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13950-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13950-1-AP

  IP result of anti-ABCF1 (IP:13950-1-AP, 4ug; Detection:13950-1-AP 1:6000) with K-562 cells lysate 1360 ug.

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  IHC staining of human stomach cancer using 13950-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 13950-1-AP

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 13950-1-AP (ABCF1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13950-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABCF1 antibody (13950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13950-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13950-1-AP (ABCF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13950-1-AP cible ABCF1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ABCF1 Protéine recombinante Ag5061
• Nom complet: ATP-binding cassette, sub-family F (GCN20), member 1
• Masse moléculaire calculée: 92 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC034488
• Symbole du gène: ABCF1
• Identification du gène (NCBI): 23
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ABCF1, also known as ABC27, and ABC50, is a member of the superfamily of ATP-binding cassette (ABC) transporters and contains two ABC transporter domains. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Unlike other members of the superfamily, ABCF1 lacks the transmembrane domains that are characteristic of most ABC transporters. ABCF1 may be regulated by tumor necrosis factor-alpha and play a role in the enhancement of protein synthesis and thus participate in the inflammatory processes(PMID: 9790762).

Publications

Species	Application	Title
	CoIP	Immunity; The ATP-Binding Cassette Gene ABCF1 Functions as an E2 Ubiquitin-Conjugating Enzyme Controlling Macrophage Polarization to Dampen Lethal Septic Shock.; Authors - Hitesh Arora
mouse,human	WB	Sci Adv; ATP-binding cassette protein ABCF1 couples transcription and genome surveillance in embryonic stem cells through low-complexity domain.; Authors - Eun-Bee Choi ; KD Validated
human	WB,IP	Proteomics; Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma; Authors - Han Hong

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