Anticorps Polyclonal de lapin anti-ABHD5

• Phare
• Validé par KD/KO

ABHD5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 12201-1-AP

Synonymes

ABHD5, CDS, CGI58, IECN2, NCIE2

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Galerie de données de validation

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  WB analysis using 12201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12201-1-AP (ABHD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12201-1-AP

  IP Result of anti-ABHD5 (IP:12201-1-AP, 4ug; Detection:12201-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human skeletal muscle using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12201-1-AP (ABHD5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12201-1-AP (ABHD5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 40x lens).

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  IHC staining of human liver using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human liver using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human liver using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human liver using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 10x lens).

• 

  IF Staining of MCF-7 using 12201-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ABHD5 antibody 12201-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HCT 116, cellules HeLa, cellules HepG2, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

12201-1-AP cible ABHD5 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ABHD5 Protéine recombinante Ag2840
• Nom complet: abhydrolase domain containing 5
• Masse moléculaire calculée: 349 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC021958
• Symbole du gène: ABHD5
• Identification du gène (NCBI): 51099
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).

Publications

Species	Application	Title
mouse	WB	Nat Commun; O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.; Authors - Yunfan Yang
mouse	WB,CoIP	Cell Death Differ; Plin2-mediated lipid droplet mobilization accelerates exit from pluripotency by lipidomic remodeling and histone acetylation.; Authors - Yi Wu
human	WB	Proc Natl Acad Sci U S A; Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover.; Authors - Olzmann James A JA
mouse	WB,IP,IF	Proc Natl Acad Sci U S A; Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis.; Authors - Satish Patel
human	WB	J Cell Biol; ORP5 localizes to ER-lipid droplet contacts and regulates the level of PI(4)P on lipid droplets.; Authors - Ximing Du
human	WB	Elife; Inhibition of intracellular lipolysis promotes human cancer cell adaptation to hypoxia.; Authors - Xiaodong Zhang