Anticorps Polyclonal de lapin anti-ABHD5

• Phare
• Validé par KD/KO

ABHD5 Polyclonal Antibody for FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

FC (Intra), ELISA

Conjugaison

PE Fluorescent Dye

N° de cat : PE-12201

Synonymes

1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase domain-containing protein 5, CDS, CGI58, EC:2.3.1.51

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Galerie de données de validation

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  FC experiment of mouse splenocytes using PE-12201

  1x10^6 mouse splenocytes were intracellularly stained with 0.1 ug PE-conjugated ABHD5 Polyclonal antibody (PE-12201)(red), or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of mouse thymocytes using PE-12201

  1x10^6 mouse thymocytes were intracellularly stained with 0.1 ug PE-conjugated ABHD5 Polyclonal antibody (PE-12201)(red), or 0.1 ug PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): splénocytes de souris, mouse thymocytes

Dilution recommandée

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.1 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-12201 cible ABHD5 dans les applications de FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ABHD5 Protéine recombinante Ag2840
• Nom complet: abhydrolase domain containing 5
• Masse moléculaire calculée: 349 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC021958
• Symbole du gène: ABHD5
• Identification du gène (NCBI): 51099
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul contiennent 0,1% de BSA.

Informations générales

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).

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