Anticorps Polyclonal de lapin anti-ABL2

• Phare
• Validé par KD/KO

ABL2 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 17693-1-AP

Synonymes

Abelson tyrosine-protein kinase 2, Abelson-related gene protein, ABLL, ARG, EC:2.7.10.2

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Galerie de données de validation

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  WB analysis using 17693-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17693-1-AP (ABL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17693-1-AP

  IP result of anti-ABL2 (IP:17693-1-AP, 4ug; Detection:17693-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human gliomas using 17693-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17693-1-AP (ABL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 17693-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17693-1-AP (ABL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

17693-1-AP cible ABL2 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ABL2 Protéine recombinante Ag11860
• Nom complet: v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)
• Masse moléculaire calculée: 1167 aa, 127 kDa
• Poids moléculaire observé: 128-140 kDa
• Numéro d’acquisition GenBank: BC065912
• Symbole du gène: ABL2
• Identification du gène (NCBI): 27
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ABL2(Abelson tyrosine-protein kinase 2) is also named as ABLL, ARG and belongs to the protein kinase superfamily. It is a membrane-anchored nonreceptor tyrosine kinase that is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity and it has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. ABL2 can be polyubiquitinated and the polyubiquitination of ABL2 leads to degradation. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 114 kDa to 128 kDa. The antibody is specific to the others except the 60 kDa isoform of ABL2.

Publications

Species	Application	Title
rat	IF	Bioact Mater; High proportion strontium-doped micro-arc oxidation coatings enhance early osseointegration of titanium in osteoporosis by anti-oxidative stress pathway.; Authors - Xinkun Shen
rat	WB	Theranostics; Non-invasive photobiomodulation treatment in an Alzheimer Disease-like transgenic rat model.; Authors - Luodan Yang
human	WB	Elife; Abelson tyrosine-protein kinase 2 regulates myoblast proliferation and controls muscle fiber length.; Authors - Jennifer K Lee ; KO Validated
human	WB,IHC,CoIP	Oncogene; Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement; Authors - Ze Zhang
human	WB	Genomics; Bone marrow mesenchymal stem cells-derived exosomal microRNA-19b-1-5p reduces proliferation and raises apoptosis of bladder cancer cells via targeting ABL2.; Authors - Dewang Fu
human	WB	Biochim Biophys Acta; Abl2 kinase phosphorylates Bi-organellar regulator MNRR1 in mitochondria, stimulating respiration.; Authors - Siddhesh Aras

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