Anticorps Polyclonal de lapin anti-ABLIM2

ABLIM2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 22433-1-AP

Synonymes

abLIM 2, ABLIM2, Actin binding LIM protein 2, KIAA1808

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Galerie de données de validation

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  WB analysis of mouse brain using 22433-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22433-1-AP (ABLIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 22433-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22433-1-AP (ABLIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22433-1-AP

  IP result of anti-ABLIM2(IP:22433-1-AP, 4ug; Detection:22433-1-AP 1:1000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of mouse skeletal muscle using 22433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22433-1-AP (ABLIM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 22433-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22433-1-AP (ABLIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skeletal muscle using 22433-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22433-1-AP (ABLIM2 Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 22433-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22433-1-AP (ABLIM2 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 22433-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 22433-1-AP (ABLIM2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 22433-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22433-1-AP (ABLIM2 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu de muscle squelettique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

22433-1-AP cible ABLIM2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ABLIM2 Protéine recombinante Ag18198
• Nom complet: actin binding LIM protein family, member 2
• Masse moléculaire calculée: 611 aa, 68 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC122567
• Symbole du gène: ABLIM2
• Identification du gène (NCBI): 84448
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (06-10-2019)

Cell lysate on the left lane expressed GFP-ABLIM2 while on the right lane was wildtype cells. Antibody recognizes the GFP tagged construct ABLIM2 really well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

ABLIM2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:22433-1-AP)