Anticorps Polyclonal de lapin anti-ACADL

ACADL Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 17442-1-AP

Synonymes

ACAD4, ACADL, LCAD

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Galerie de données de validation

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  WB analysis using 17442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17442-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 17442-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17442-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17442-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17442-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17442-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17442-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17442-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 17442-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17442-1-AP (ACADL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 17442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17442-1-AP (ACADL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17442-1-AP (ACADL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17442-1-AP (ACADL Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 17442-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 17442-1-AP (ACADL Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, cellules HeLa, cellules HepG2, cellules NIH/3T3, tissu rénal de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

17442-1-AP cible ACADL dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACADL Protéine recombinante Ag11530
• Nom complet: acyl-Coenzyme A dehydrogenase, long chain
• Masse moléculaire calculée: 430 aa, 48 kDa
• Poids moléculaire observé: 45-48 kDa
• Numéro d’acquisition GenBank: BC039063
• Symbole du gène: ACADL
• Identification du gène (NCBI): 33
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).

Publications

Species	Application	Title
human	WB	Nat Commun; Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients.; Authors - Ya-Kun Zhang
rat	WB	Front Pharmacol; Lipidomics reveals the potential mechanism of honokiol against adenine-induced chronic kidney disease; Authors - Xinhui Liu
mouse	WB	Front Pharmacol; The serum acylcarnitines profile in epileptic children treated with valproic acid and the protective roles of peroxisome proliferator-activated receptor a activation in valproic acid-induced liver injury; Authors - Yiyi Ma
human,mouse	WB	Diabetologia; Chaperonin counteracts diet-induced non-alcoholic fatty liver disease by aiding sirtuin 3 in the control of fatty acid oxidation; Authors - Shao-Wen Weng