Anticorps Polyclonal de lapin anti-ACADL-Specific

• Phare
• Validé par KD/KO

ACADL-Specific Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

44

Avis (2)

N° de cat : 17526-1-AP

Synonymes

ACAD4, ACADL, ACADL-Specific, LCAD

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Galerie de données de validation

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  WB analysis using 17526-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17526-1-AP (ACADL-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17526-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17526-1-AP (ACADL-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 17526-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17526-1-AP (ACADL-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17526-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17526-1-AP (ACADL-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 17526-1-AP

  IP result of anti-ACADL-Specific(IP:17526-1-AP, 4ug; Detection:17526-1-AP 1:10000) with mouse kidney tissue lysate 2160 ug.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu cardiaque de souris, tissu hépatique de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 39 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• IP: See 3 publications below

Informations sur le produit

17526-1-AP cible ACADL-Specific dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris, Brebis
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: acyl-Coenzyme A dehydrogenase, long chain
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: NM_001608
• Symbole du gène: ACADL
• Identification du gène (NCBI): 33
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).This antibody is specific to ACADL.

Publications

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
mouse	WB	Circ Res; NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.; Authors - Dan Tong
human	WB,IF	Int J Biol Sci; Bulk and single-cell transcriptome profiling reveal extracellular matrix mechanical regulation of lipid metabolism reprograming through YAP/TEAD4/ACADL axis in hepatocellular carcinoma; Authors - Jingwei Cai
mouse	WB	Diabetes; Pivotal Role of O-GlcNAc Modification in Cold-Induced Thermogenesis by Brown Adipose Tissue Through Mitochondrial Biogenesis.; Authors - Natsuko Ohashi
mouse, human	WB, IHC	Cell Rep; HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.; Authors - De Huang ; KD Validated
mouse	WB	Cell Mol Gastroenterol Hepatol; Fatty Acids Inhibit LAMP2-Mediated Autophagy Flux via Activating ER Stress Pathway in Alcohol-Related Liver Disease.; Authors - Wei Guo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wu (Verified Customer) (04-10-2021)

LCAD antibody worked good in IF assay at 1:50 dilution.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Liver

ACADL-Specific Antibody Immunofluorescence validation (1:50 dilution) in Liver (Cat no:17526-1-AP)

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain