Anticorps Polyclonal de lapin anti-ACADM

• Phare
• Validé par KD/KO

ACADM Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(61)

Avis (2)

N° de cat : 55210-1-AP

Synonymes

ACAD, ACAD 1, ACAD1, EC:1.3.8.7, MCAD

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Galerie de données de validation

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  WB analysis using 55210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 55210-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 55210-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55210-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55210-1-AP (ACADM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 55210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55210-1-AP (ACADM antibody) at dilution of 1:150 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 55210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55210-1-AP (ACADM antibody) at dilution of 1:150 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 55210-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55210-1-AP (ACADM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55210-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55210-1-AP (ACADM antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules K-562, cellules MDA-MB-453s, tissu cardiaque de rat, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 57 publications below
• IHC: See 8 publications below
• IP: See 1 publications below

Informations sur le produit

55210-1-AP cible ACADM dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: NM_000016
• Symbole du gène: ACADM
• Identification du gène (NCBI): 34
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

Publications

Species	Application	Title
mouse	WB	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
mouse	IHC	Nat Cell Biol; BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.; Authors - Jin-Tao Li
mouse	WB	Circ Res; NAD+ Repletion Reverses Heart Failure With Preserved Ejection Fraction.; Authors - Dan Tong
mouse	WB	Redox Biol; Increased fatty acid metabolism attenuates cardiac resistance to β-adrenoceptor activation via mitochondrial reactive oxygen species: A potential mechanism of hypoglycemia-induced myocardial injury in diabetes.; Authors - Lishan Huang
mouse	WB	Cell Biosci; Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy; Authors - Mei-Fei Wu
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yasuyo (Verified Customer) (07-22-2021)

Works good consistently.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: liver

ACADM Antibody Immunohistochemistry validation (1:150 dilution) in liver (Cat no:55210-1-AP)

FH

chao (Verified Customer) (01-30-2020)

Single strong, clear band for heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

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