Anticorps Polyclonal de lapin anti-ACADSB

• Phare
• Validé par KD/KO

ACADSB Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 13122-1-AP

Synonymes

2 MEBCAD, ACAD7, ACADSB, SBCAD

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Galerie de données de validation

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  WB analysis of HepG2 using 13122-1-AP

  WB result of ACADSB antibody (13122-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACADSB transfected HepG2 cells.

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  WB analysis of mouse kidney using 13122-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13122-1-AP (ACADSB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13122-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13122-1-AP (ACADSB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 13122-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13122-1-AP (ACADSB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13122-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13122-1-AP (ACADSB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

13122-1-AP cible ACADSB dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACADSB Protéine recombinante Ag3851
• Nom complet: acyl-Coenzyme A dehydrogenase, short/branched chain
• Masse moléculaire calculée: 432 aa, 47 kDa
• Poids moléculaire observé: 45-47 kDa
• Numéro d’acquisition GenBank: BC013756
• Symbole du gène: ACADSB
• Identification du gène (NCBI): 36
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery; Authors - Jasmin Adriana Schafer
mouse	WB	Cell Mol Life Sci; Mitochondrial FAD shortage in SLC25A32 deficiency affects folate-mediated one-carbon metabolism.; Authors - Min-Zhi Peng
human	IHC	Front Oncol; Decreased Expression of ACADSB Predicts Poor Prognosis in Clear Cell Renal Cell Carcinoma.; Authors - Xianhui Liu
human	IHC,IF	Cell Biol Int; ACADSB regulates ferroptosis and affects the migration, invasion, and proliferation of colorectal cancer cells.; Authors - Di Lu
human	IHC	Nat Commun; AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis; Authors - Yanan Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Süleyman (Verified Customer) (02-06-2022)

I used HeLa whole cell extract with Licor secondary antibody. It recognize possible ACADSB but I also get many unspecific bands especially around 70 kDa much specific binding.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

ACADSB Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:13122-1-AP)