Anticorps Polyclonal de lapin anti-ACE2

• Phare
• Validé par KD/KO

ACE2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

109

Avis (2)

N° de cat : 21115-1-AP

Synonymes

ACE related carboxypeptidase, ACE2, ACEH, Angiotensin converting enzyme 2, Angiotensin I converting enzyme 2, Metalloprotease MPROT15

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Galerie de données de validation

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  WB analysis using 21115-1-AP

  mouse kidney tissue lysates were subjected to SDS PAGE followed by western blot with 21115-1-AP (ACE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 21115-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21115-1-AP (ACE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 21115-1-AP

  IP Result of anti-ACE2 (IP:21115-1-AP, 5ug; Detection:21115-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human kidney using 21115-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (21115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), PTPRO antibody (67000-1-Ig, Clone: 2F2B4, red).

• 

  IF Staining of human kidney using 21115-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (21115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), PTPRO antibody (67000-1-Ig, Clone: 2F2B4, red).

• 

  IF Staining of mouse kidney using 21115-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ACE2 antibody (21115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse kidney using 21115-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ACE2 antibody (21115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 21115-1-AP

  1X10^6 NIH/3T3 cells were stained with 0.20 ug/test Anti-Human ACE2 (21115-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu de muscle squelettique humain
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, tissu d'intestin grêle humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu rénal humain, tissu rénal de souris
• Résultats positifs en cytométrie: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 79 publications below
• IHC: See 21 publications below
• IF: See 22 publications below
• IP: See 3 publications below
• FC: See 7 publications below
• CoIP: See 2 publications below

Informations sur le produit

21115-1-AP cible ACE2 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACE2 Protéine recombinante Ag15455
• Nom complet: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Masse moléculaire calculée: 805 aa, 92 kDa
• Poids moléculaire observé: 120 kDa, 92 kDa
• Numéro d’acquisition GenBank: BC048094
• Symbole du gène: ACE2
• Identification du gène (NCBI): 59272
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).

Publications

Species	Application	Title
human	WB	Nat Genet; Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs; Authors - Antoine Rebendenne
human	IF	Cell Stem Cell; Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men.; Authors - Ryan M Samuel
human,mouse	IF	Cell Res; Receptome profiling identifies KREMEN1 and ASGR1 as alternative functional receptors of SARS-CoV-2.; Authors - Yunqing Gu
human	IHC	Nat Neurosci; Olfactory transmucosal SARS-CoV-2 invasion as a port of central nervous system entry in individuals with COVID-19.; Authors - Jenny Meinhardt
human	WB,FC	Mol Cell; Enhanced SARS-CoV-2 entry via UPR-dependent AMPK-related kinase NUAK2; Authors - Vibhu Prasad
human	WB	Eur Respir J; Influenza virus infection increases ACE2 expression and shedding in human small airway epithelial cells.; Authors - Kelly S Schweitzer

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Magdalena (Verified Customer) (07-18-2022)

I'm satisfied with this antibody. I used this antibody in dot-blot technique and IF. In IF I used dilution factor 1:200, seconday antibody was alexa fluor 555.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:200

FH

Ratna (Verified Customer) (01-14-2021)

Good

• Applications: Western Blot