- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-ACE2
ACE2 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, souris et plus (1)
Applications
WB, IHC, IF-P, IP, ELISA
Conjugaison
Non conjugué
CloneNo.
2F12A4
N° de cat : 66699-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | tissu testiculaire humain, tissu de muscle squelettique humain |
| Résultats positifs en IHC | tissu d'intestin grêle humain, tissu de cancer du côlon humain, tissu de côlon humain, tissu rénal humain, tissu testiculaire humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF-P | tissu rénal humain, tissu cardiaque de souris, tissu testiculaire de souris |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:6000 |
| Immunohistochimie (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 1 publications below |
| WB | See 25 publications below |
| IHC | See 5 publications below |
| IF | See 12 publications below |
| IP | See 1 publications below |
| FC | See 1 publications below |
Informations sur le produit
66699-1-Ig cible ACE2 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris
| Réactivité | Humain, souris |
| Réactivité citée | Humain, singe, souris |
| Hôte / Isotype | Mouse / IgG1 |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | ACE2 Protéine recombinante Ag15554 |
| Nom complet | angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 |
| Masse moléculaire calculée | 805 aa, 92 kDa |
| Poids moléculaire observé | 120 kDa, 92 kDa |
| Numéro d’acquisition GenBank | BC048094 |
| Symbole du gène | ACE2 |
| Identification du gène (NCBI) | 59272 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine G |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for ACE2 antibody 66699-1-Ig | Download protocol |
| IHC protocol for ACE2 antibody 66699-1-Ig | Download protocol |
| IF protocol for ACE2 antibody 66699-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men. | ||
Ocul Surf SARS-CoV-2 receptor ACE2 is expressed in human conjunctival tissue, especially in diseased conjunctival tissue. | ||
Cell Death Differ SARS-CoV-2 spike protein dictates syncytium-mediated lymphocyte elimination. | ||
J Control Release Peritoneal M2 macrophage-derived extracellular vesicles as natural multitarget nanotherapeutics to attenuate cytokine storms after severe infections. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Lindsey (Verified Customer) (05-24-2021) | Great antibody---looking forward to trying the RabPolyAb as well
 |
FH Lindsey (Verified Customer) (09-21-2020) | Perfomed IF analysis on human FFPE and mouse frozen tissue sections; for FFPE tissue, antigen retrieval was performed for 30 min at 95C using Tris EDTA Buffer (pH9.0); Permeabilization was not performed; Blocking was performed using 5% Normal Serum and 0.3% TritonX-100 for 30. minutes at room temperature. Mouse lung sections were costained with ARL13B (Proteintech #1711-1-AP) to visualize primary cilia of the lung epithelia; all sections were counterstained with DAPI Fluormount (Abcam); image attached is of mouse lung frozen tissue; due to size restrictions I am limited to this image only but am happy to provide additional images (human tissue etc.) upon request
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