Anticorps Monoclonal anti-ACE2

• Phare
• Validé par KD/KO

ACE2 Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris et plus (1)

Applications

IF-P

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(3)

CloneNo.

2F12A4

N° de cat : CL488-66699

Synonymes

ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15

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Galerie de données de validation

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  IF Staining of human kidney using CL488-66699

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 ACE2 antibody (CL488-66699, Clone: 2F12A4 ) at dilution of 1:200.

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  IF Staining of human kidney using CL488-66699

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite® Plus 488 ACE2 antibody (CL488-66699, Clone: 2F12A4 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF-P: tissu rénal humain,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below
• FC: See 1 publications below

Informations sur le produit

CL488-66699 cible ACE2 dans les applications de IF-P et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACE2 Protéine recombinante Ag15554
• Nom complet: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Masse moléculaire calculée: 805 aa, 92 kDa
• Numéro d’acquisition GenBank: BC048094
• Symbole du gène: ACE2
• Identification du gène (NCBI): 59272
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2.

Publications

Species	Application	Title
	FC	Adv Mater; Engineering Extracellular Vesicles Enriched with Palmitoylated ACE2 as COVID-19 Therapy.; Authors - Feng Xie
human	IF	J Virol; The "LLQY" motif on SARS-CoV-2 spike protein affects S incorporation into virus particles.; Authors - Shouwen Du
rat	IF	Mol Med Rep; Swimming training promotes angiogenesis of endothelial progenitor cells by upregulating IGF1 expression and activating the PI3K/AKT pathway in type 2 diabetic rats; Authors - Lan Li

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