Anticorps Polyclonal de lapin anti-Acetylcholinesterase

Acetylcholinesterase Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (2)

N° de cat : 17975-1-AP

Synonymes

Acetylcholinesterase, ACHE, ARACHE, N ACHE, YT

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Galerie de données de validation

• 

  WB analysis of mouse liver using 17975-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17975-1-AP (Acetylcholinesterase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17975-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17975-1-AP (Acetylcholinesterase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 17975-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17975-1-AP (Acetylcholinesterase antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 17975-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17975-1-AP (Acetylcholinesterase antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HEK-293
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

17975-1-AP cible Acetylcholinesterase dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Acetylcholinesterase Protéine recombinante Ag12146
• Nom complet: acetylcholinesterase (Yt blood group)
• Masse moléculaire calculée: 614aa,68 kDa; 640aa,71 kDa
• Poids moléculaire observé: 66-72 kDa
• Numéro d’acquisition GenBank: BC094752
• Symbole du gène: ACHE
• Identification du gène (NCBI): 43
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The classical role of acetylcholinesterase (AChE) is to terminate cholinergic neurotransmission by rapid hydrolysis of the neurotransmitter acetylcholine (ACh) at cholinergic synapses and neuromuscular junctions(PMID:20054652). AChE activity is significantly lower in aspirates from squamous cell carcinomas and the molecular distribution of AChE in both bronchial cells and fluids consisted of amphiphilic monomers and dimers(PMID:18211261). It has 4 isoforms produced by alternative splicing.

Publications

Species	Application	Title
rat	WB	ACS Sens; Near-Infrared Fluorescence Probe for Evaluating Acetylcholinesterase Activity in PC12 Cells and In Situ Tracing AChE Distribution in Zebrafish.; Authors - Jianlong Ma
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
mouse	WB	Transl Psychiatry; A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.; Authors - Yinrui Guo
mouse	IHC	Front Pharmacol; Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice.; Authors - Yang Yang
		J Alzheimers Dis; Lithium Attenuates Scopolamine-Induced Memory Deficits with Inhibition of GSK-3β and Preservation of Postsynaptic Components.; Authors - Wu Yuan-Yuan YY

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Zulaikha (Verified Customer) (09-21-2022)

Consistent and reproducible results.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue

FH

Dr Shane (Verified Customer) (08-17-2020)

Very good antibody and worked ok.

• Applications: Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Cell culture
• Cell Tissue Type: Chemical combination