Anticorps Polyclonal de lapin anti-ACP1

ACP1 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 22214-1-AP

Synonymes

acid phosphatase 1, soluble, ACP1, Adipocyte acid phosphatase, HAAP, LMW PTP, LMW PTPase, Red cell acid phosphatase 1

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Galerie de données de validation

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  WB analysis of mouse brain using 22214-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22214-1-AP (ACP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22214-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22214-1-AP (ACP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of 4T1 using 22214-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 22214-1-AP (ACP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 22214-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22214-1-AP (ACP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 22214-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22214-1-AP (ACP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 22214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22214-1-AP (ACP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22214-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22214-1-AP (ACP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22214-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22214-1-AP (ACP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22214-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22214-1-AP (ACP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules 4T1
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

22214-1-AP cible ACP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACP1 Protéine recombinante Ag17539
• Nom complet: acid phosphatase 1, soluble
• Masse moléculaire calculée: 158 aa, 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC007422
• Symbole du gène: ACP1
• Identification du gène (NCBI): 52
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human red cell acid phosphatase (ACP1) is a polymorphic enzyme closely related to cytosolic low molecular weight acid phosphatases, a protein family broadly conserved among eukaryotes. It catalyses the transfer of phosphate from phosphate ester substrates to suitable acceptor alcohols such as methanol and glycerol. This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	IF	Nat Commun; Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules; Authors - Tao Wang