Anticorps Monoclonal anti-ACSL4

• Phare
• Validé par KD/KO

ACSL4 Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

11

CloneNo.

1H5D3

N° de cat : 66617-1-Ig

Synonymes

ACS4, ACSL4, FACL4, LACS 4, LACS4, MRX63, MRX68

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Galerie de données de validation

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  WB analysis using 66617-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66617-1-Ig

  WB result of ACSL4 antibody (66617-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells.

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  WB analysis of HeLa using 66617-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66617-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 66617-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 66617-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66617-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66617-1-Ig (ACSL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cerebellum using 66617-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human cerebellum using 66617-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66617-1-Ig (ACSL4 antibody) at dilution of 1:500 (under 40x lens).

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  FC experiment of HEK-293T using 66617-1-Ig

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human ACSL4 (66617-1-Ig, Clone:1H5D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Caco-2, cellules COLO 320, cellules HepG2, cellules HT-29, cellules L02
• Résultats positifs en IHC: tissu de cervelet humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HEK-293T,

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below

Informations sur le produit

66617-1-Ig cible ACSL4 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ACSL4 Protéine recombinante Ag18085
• Nom complet: acyl-CoA synthetase long-chain family member 4
• Masse moléculaire calculée: 711 aa, 79 kDa
• Poids moléculaire observé: 74 kDa
• Numéro d’acquisition GenBank: BC034959
• Symbole du gène: ACSL4
• Identification du gène (NCBI): 2182
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Free Radic Biol Med; Targeting the Na+/K+ ATPase DR-region with DR-Ab improves doxorubicin-induced cardiotoxicity; Authors - Bin Leng
human	WB	J Med Chem; Seco-Lupane Triterpene Derivatives Induce Ferroptosis through GPX4/ACSL4 Axis and Target Cyclin D1 to Block the Cell Cycle.; Authors - Haohao Wang
human	WB	Front Pharmacol; Targeting Mitochondrial ROS-Mediated Ferroptosis by Quercetin Alleviates High-Fat Diet-Induced Hepatic Lipotoxicity.; Authors - Jin-Jin Jiang
human	WB	Ann Transl Med; Atractylodin may induce ferroptosis of human hepatocellular carcinoma cells.; Authors - Yongfei He
rat	WB	Cell Stress Chaperones; Activation of NRF2/FPN1 pathway attenuates myocardial ischemia-reperfusion injury in diabetic rats by regulating iron homeostasis and ferroptosis.; Authors - Hao Tian
	WB	FEBS Open Bio; Lipoxin A4 protects primary spinal cord neurons from Erastin-induced ferroptosis by activating the Akt/Nrf2/HO-1 signaling pathway.; Authors - Na Wei