Anticorps Recombinant de lapin anti-ACSS1

• Phare
• Validé par KD/KO

ACSS1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242361C6

N° de cat : 85102-4-RR

Synonymes

242361C6, ACAS2L, Acetate--CoA ligase 2, Acetyl-CoA synthetase 2, Acetyl-coenzyme A synthetase 2-like, mitochondrial

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Galerie de données de validation

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  WB analysis using 85102-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85102-4-RR (ACSS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85102-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85102-4-RR (ACSS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 85102-4-RR

  WB result of ACSS1 antibody (85102-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSS1 transfected HepG2 cells.

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  FC experiment of HeLa using 85102-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug ACSS1 Recombinant antibody (85102-4-RR, Clone:242361C6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Caco-2, cellules Jurkat, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85102-4-RR cible ACSS1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ACSS1 Protéine recombinante Ag10853
• Nom complet: acyl-CoA synthetase short-chain family member 1
• Masse moléculaire calculée: 689 aa, 75 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC039261
• Symbole du gène: ACSS1
• Identification du gène (NCBI): 84532
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ACSS (acetyl-CoA synthetase) enzyme is the sole known mammalian enzyme that can catalyze the conversion of free acetate into acetyl coenzyme A (acetyl-CoA). The three known isoforms of human ACSS are termed ACSS1, ACSS2, and ACSS3. The main substrate of ACSS1 and ACSS2 is acetate, while the preferential substrate of ACSS3 is propionate. Two acetate related enzymes, ACSS1(GeneID: 84532) and ACSS2 (GeneID:55902) difer in their tissue distribution and subcellular localization. On the one hand, as a mitochondrial matrix enzyme, ACSS1 is expressed mainly in cardiac and skeletal muscle as well as brown adipose tissue. On the other hand, as a nuclear and cytoplasmic enzyme, ACSS2 is strongly expressed in the liver, kidney and heart and moderately expressed in the brain and testis.ACSS2 participates in lipid synthesis and facilitates protein acetylation by generating acetyl-CoA, while ACSS1 is involved in acetate oxidation. The functional diferences in these enzymes involve energy production through the tricarboxylic acid (TCA) cycle. Due to its more thorough utilization of intracellular acetate, ACSS2 is expressed in almost all cell types under diferent physiological conditions.

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