Anticorps Polyclonal de lapin anti-ACSS3

• Phare
• Validé par KD/KO

ACSS3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 16204-1-AP

Synonymes

EC:6.2.1.17, EC:6.2.1.1, Acyl-CoA synthetase short-chain family member 3, mitochondrial, Acyl-CoA synthetase short-chain family member 3, ACSS 3

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Galerie de données de validation

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  WB analysis using 16204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16204-1-AP (ACSS3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16204-1-AP

  WB result of ACSS3 antibody (16204-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSS3 transfected HepG2 cells.

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  IHC staining of human liver using 16204-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16204-1-AP (ACSS3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16204-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16204-1-AP (ACSS3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of U2OS using 16204-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ACSS3 antibody (16204-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of U2OS using 16204-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ACSS3 antibody (16204-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 2 publications below

Informations sur le produit

16204-1-AP cible ACSS3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACSS3 Protéine recombinante Ag9173
• Nom complet: acyl-CoA synthetase short-chain family member 3
• Masse moléculaire calculée: 686 aa, 75 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC009317
• Symbole du gène: ACSS3
• Identification du gène (NCBI): 79611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACSS3(acyl-CoA synthetase short-chain family member 3, mitochondrial) belongs to the ATP-dependent AMP-binding enzyme family. It activates acetate so that it can be used for lipid synthesis or for energy generation. The deduced 686-amino acid protein contains 4 of 5 motifs characteristic of acyl-CoA synthetases. This protein has 2 isoforms produced by alternative splicing. The full length protein has a transit peptide with 29 amino acids.

Publications

Species	Application	Title
	WB	Cell Metab; Amelioration of nonalcoholic fatty liver disease by inhibiting the deubiquitylating enzyme RPN11; Authors - Bing Zhou
human,mouse	WB,IHC	Cancer Commun (Lond); Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma; Authors - Xiaoteng Cui
	WB	Nat Commun; ACSS2 protects against alcohol-induced hepatocyte ferroptosis through regulation of hepcidin expression; Authors - Mengyao Wang
mouse	WB	Nutrients; Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent.; Authors - Karolin Weitkunat
human,mouse	WB	Biochem J; NRF2/ACSS2 axis mediates the metabolic effect of alcohol drinking on esophageal squamous cell carcinoma.; Authors - Joab Otieno Odera
human	WB	Cancer Genet; Prognosis prediction and drug guidance of ovarian serous cystadenocarcinoma through mitochondria gene-based model; Authors - Dongsheng Shen

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