Anticorps Polyclonal de lapin anti-smooth muscle actin

smooth muscle actin Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 23081-1-AP

Synonymes

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, ACTA2 specific, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, smooth muscle actin, α SMA, α-sma

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Galerie de données de validation

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  WB analysis of mouse heart using 23081-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brown adipose using 23081-1-AP

  mouse brown adipose tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (a-SMA specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 23081-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 23081-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 23081-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 23081-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 23081-1-AP

  IP Result of anti-a-SMA specific (IP:23081-1-AP, 4ug; Detection:23081-1-AP 1:1000) with mouse heart tissue lysate 3200ug.

• 

  IHC staining of human heart using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human colon using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human liver cancer using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of stromal tumor using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 10x lens).

• 

  IHC staining of stromal tumor using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded stromal tumor tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 40x lens).

• 

  IHC staining of mouse skeletal muscle using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human small intestine using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human hysteromyoma using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hysteromyoma using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 23081-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 23081-1-AP (ACTA2/smooth muscle actin antibody) at dilution of 1:500 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu adipeux brun de souris, tissu cardiaque de rat, tissu de muscle squelettique de souris, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu de cancer du foie humain, tissu de côlon humain, tissu de muscle squelettique de souris, tissu de muscle squelettique humain, tissu d'hystéromyome humain, tissu d'intestin grêle humain, tissu hépatique humain, tissu stroma tumoral; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 11 publications below
• IF: See 6 publications below

Informations sur le produit

23081-1-AP cible smooth muscle actin dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: smooth muscle actin Protéine recombinante Ag19481
• Nom complet: actin, alpha 2, smooth muscle, aorta
• Masse moléculaire calculée: 377 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC017554
• Symbole du gène: ACTA2
• Identification du gène (NCBI): 59
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody is specific to the ACTA2. It selectively stains the vascular smooth muscle but not cardiac or skeletal muscle.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Dental cell type atlas reveals stem and differentiated cell types in mouse and human teeth.; Authors - Jan Krivanek
human	WB	Drug Des Devel Ther; Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling; Authors - Huan Hu
mouse	IHC	Cardiovasc Res; Plasma levels of trimethylamine-N-oxide can be increased with 'healthy' and 'unhealthy' diets and do not correlate with the extent of atherosclerosis but with plaque instability.; Authors - Yen Chin Koay
rat	WB	Environ Pollut; Polystyrene microplastics cause cardiac fibrosis by activating Wnt/β-catenin signaling pathway and promoting cardiomyocyte apoptosis in rats.; Authors - Zekang Li
rat	IHC	Cardiovasc Res; PMID: 23650288; Authors - Fan You-Fei YF
mouse	IHC	Int J Mol Sci; SIRT2 Deficiency Aggravates Diet-Induced Nonalcoholic Fatty Liver Disease through Modulating Gut Microbiota and Metabolites; Authors - Xingyu Li

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