Anticorps Polyclonal de lapin anti-smooth muscle actin specific

smooth muscle actin specific Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

229

Avis (2)

N° de cat : 55135-1-AP

Synonymes

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, ACTA2 Smooth muscle Actin Specific, Actin, aortic smooth muscle, Alpha actin 2, Alpha SMA, Alpha SMA Specific, a-SMA, smooth muscle actin, smooth muscle actin-specific, α SMA, α-sma

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Galerie de données de validation

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  WB analysis using 55135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55135-1-AP (smooth muscle actin-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 55135-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55135-1-AP (smooth muscle actin specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 10x lens).

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  IHC staining of human colon cancer using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 40x lens).

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  IHC staining of human colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 10x lens).

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  IHC staining of human colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:2000 (under 40x lens).

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  IHC staining of rat colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of rat colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of mouse colon using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:2000 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 55135-1-AP (ACTA2-Specific antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse liver using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 55135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 55135-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 55135-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 55135-1-AP (Alpha SMA-specific antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de côlon de souris, côlon de rat, poumon de rat, poumon de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de cirrhose hépatique humain, tissu de côlon de rat, tissu de côlon de souris, tissu de côlon humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cardiaque de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 153 publications below
• IHC: See 58 publications below
• IF: See 98 publications below

Informations sur le produit

55135-1-AP cible smooth muscle actin specific dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: actin, alpha 2, smooth muscle, aorta
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: NM_001613
• Symbole du gène: ACTA2
• Identification du gène (NCBI): 59
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.

Publications

Species	Application	Title
mouse	IF	ACS Nano; Cysteine-Based Redox-Responsive Nanoparticles for Fibroblast-Targeted Drug Delivery in the Treatment of Myocardial Infarction; Authors - Xiaoqian Ji
human	IHC	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
mouse	IF	Nat Commun; Copper sulfide nanoparticles as a photothermal switch for TRPV1 signaling to attenuate atherosclerosis.; Authors - Wen Gao
human	IF	Mol Cancer; Extracellular vesicles of carcinoma-associated fibroblasts creates a pre-metastatic niche in the lung through activating fibroblasts.; Authors - Jing Kong
mouse	WB,IF	J Nanobiotechnology; Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy; Authors - Zhenxian Li
human,mouse	WB,IF	Aging Dis; HIF-1α promotes the migration and invasion of cancer-associated fibroblasts by miR-210; Authors - Ying Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

PK (Verified Customer) (03-22-2022)

• Applications: Western Blot
• Cell Tissue Type: cardiac Fibroblast

smooth muscle actin specific Antibody Western Blot validation ( dilution) in cardiac Fibroblast (Cat no:55135-1-AP)

FH

S (Verified Customer) (12-01-2021)

Excellent

• Applications: Western Blot
• Cell Tissue Type: LUNG FIBROBLASTS

smooth muscle actin specific Antibody Western Blot validation ( dilution) in LUNG FIBROBLASTS (Cat no:55135-1-AP)

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