Anticorps Monoclonal anti-Beta Actin

Beta Actin Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, plante, poisson-zèbre, rat, souris et plus (4)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

2929

CloneNo.

7D2C10

Avis (5)

N° de cat : 60008-1-Ig

Synonymes

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, β actin

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Galerie de données de validation

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  WB analysis of multi-cells/tissue using 60008-1-Ig

  Western blot analysis of Beta-actin in various tissues and cell lines using Proteintech antibody 60008-1-Ig at a dilution of 1:5000. Extra bands were detected in some species with unknown reason.

• 

  WB analysis of HeLa cells using 60008-1-Ig

  Western blot of Hela cell with anti-Actin-Beta (60008-1-Ig) at various dilutions.

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  WB analysis of arabidopsis whole plant using 60008-1-Ig

  arabidopsis whole plant tissue were subjected to SDS PAGE followed by western blot with 60008-1-Ig (beta actin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60008-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60008-1-Ig (ACTB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60008-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60008-1-Ig (ACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 60008-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60008-1-Ig (ACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 60008-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 60008-1-Ig (ACTB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rice whole plant using 60008-1-Ig

  rice whole plant tissue were subjected to SDS PAGE followed by western blot with 60008-1-Ig (beta Actin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60008-1-Ig(ACTB antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60008-1-Ig(ACTB antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart slide using 60008-1-Ig (beta actin Antibody) at dilution of 1:50.

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  IHC staining of human ovary using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60008-1-Ig (beta Actin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60008-1-Ig (beta Actin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart slide using 60008-1-Ig (beta actin Antibody) at dilution of 1:50.

• 

  IHC staining of human placenta using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 60008-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung using 60008-1-Ig(ACTB antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 60008-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60008-1-Ig (beta actin Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-mouse IgG (red).

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  FC experiment of HepG2 using 60008-1-Ig

  1X10^6 HepG2 cells were stained with 0.2ug beta actin antibody (60008-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: multi-cellules/tissus, cellules A549, cellules HEK-293, cellules HeLa, cellules MCF-7, tissu végétal entier d'arabidopsis, tissu végétal entier de riz
• Résultats positifs en IHC: tissu de côlon humain, tissu cardiaque humain, tissu cérébral humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2654 publications below
• IHC: See 4 publications below
• IF: See 8 publications below
• IP: See 6 publications below
• CoIP: See 2 publications below

Informations sur le produit

60008-1-Ig cible Beta Actin dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, plante, poisson-zèbre, rat, souris

• Réactivité: Humain, plante, poisson-zèbre, rat, souris
• Réactivité citée: rat, Chèvre, Humain, levure, poulet, arabidopsis
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Beta Actin Protéine recombinante Ag0297
• Nom complet: actin, beta
• Masse moléculaire calculée: 375 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC002409
• Symbole du gène: ACTB
• Identification du gène (NCBI): 60
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Publications

Species	Application	Title
human	WB,CoIP	Cell Res; Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression; Authors - Shiqiao Qiu
	WB	Nature; A phase-separated nuclear GBPL circuit controls immunity in plants.; Authors - Shuai Huang
human	WB	Gastroenterology; PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis; Authors - Tian-Yi Jiang
human	IF	Nat Methods; Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).; Authors - Marine H Laporte
human	WB	Cell Metab; Microenvironmental ammonia enhances T cell exhaustion in colorectal cancer; Authors - Hannah N Bell
human	WB	Nat Genet; Simultaneous disruption of PRC2 and enhancer function underlies histone H3.3-K27M oncogenic activity in human hindbrain neural stem cells.; Authors - Gerard L Brien

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

The Ab worked well for both WB and IF at mentioned dilutions

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 for WB and 1:200 for IF
• Cell Tissue Type: Glioblastoma cells

FH

Chun (Verified Customer) (09-07-2020)

This is an excellent antibody for immunoblotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293, HeLa, HaCaT and SH-SY5Y cells

FH

Nikhil (Verified Customer) (09-17-2019)

Very good and specific. I can use 1:2500 5 ml antibody solution two times.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: Colon cancer cells

Beta Actin Antibody Western Blot, validation (1:2500 dilution) in Colon cancer cells (Cat no:60008-1-Ig)

FH

Leonardo (Verified Customer) (09-12-2019)

Excellent antibody. Works well since the first time.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5,000
• Cell Tissue Type: Human breast cancer (MDA-MB-231)

Beta Actin Antibody Western Blot, validation (1:5,000 dilution) in Human breast cancer (MDA-MB-231) (Cat no:60008-1-Ig)

FH

mark (Verified Customer) (02-05-2019)

Antibody works beautifully to detect a single Beta-actin band as a control

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: human cancer cells

Beta Actin Antibody Western Blot, validation (1:2000 dilution) in human cancer cells (Cat no:60008-1-Ig)

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