Anticorps Polyclonal de lapin anti-ACTL7B

ACTL7B Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 13537-1-AP

Synonymes

Actin like 7 beta, actin like 7B, Actin like protein 7B, ACTL7B

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Galerie de données de validation

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  WB analysis of human testis using 13537-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13537-1-AP (ACTL7B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13537-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13537-1-AP (ACTL7B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13537-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13537-1-AP (ACTL7B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 13537-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13537-1-AP (ACTL7B antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 13537-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13537-1-AP (ACTL7B antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, tissu hépatique humain, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

13537-1-AP cible ACTL7B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACTL7B Protéine recombinante Ag4460
• Nom complet: actin-like 7B
• Masse moléculaire calculée: 415 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC033789
• Symbole du gène: ACTL7B
• Identification du gène (NCBI): 10880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACTL7B is also named as actin-like-7-beta. The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. The ACTL7B gene is expressed predominantly in the testis, however, its exact function is not known. Diseases associated with ACTL7B include Dysautonomia and Hypertensive Nephropathy. An important paralog of this gene is ACTL7A. ACTL7B can be detected as 45 kDa.

Publications

Species	Application	Title
		Cell Rep; The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids.; Authors - Brian P Hermann
human,mouse	WB	Development; Actl7b deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis; Authors - Gina E Merges