Anticorps Polyclonal de lapin anti-Alpha Actinin

• Phare
• Validé par KD/KO

Alpha Actinin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(50)

Avis (2)

N° de cat : 11313-2-AP

Synonymes

ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1

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Galerie de données de validation

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  WB analysis using 11313-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11313-2-AP (Alpha Actinin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11313-2-AP

  WB result of Alpha Actinin antibody (11313-2-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Actinin transfected HeLa cells.

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  WB analysis of rat heart using 11313-2-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11313-2-AP (alpha Actinin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 11313-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11313-2-AP (alpha Actinin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 11313-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 11313-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 11313-2-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11313-2-AP (Alpha Actinin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 11313-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 11313-2-AP (alpha Actinin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66219-1-Ig (N-cadherin) in red.

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  IF Staining of mouse heart using 11313-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed mouse heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse skeletal muscle using 11313-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse skeletal muscle using 11313-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat heart using 11313-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat heart using 11313-2-AP

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HSkMC using 11313-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HSkMC cells using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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  FC experiment of A431 using 11313-2-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actinin (11313-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules C6, cellules HeLa, tissu cardiaque de rat, tissu splénique de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de rat, tissu cardiaque de souris, tissu de muscle squelettique de souris
• Résultats positifs en IF/ICC: HSkMC cells,
• Résultats positifs en FC (Intra): cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 28 publications below
• IHC: See 2 publications below
• IF: See 18 publications below

Informations sur le produit

11313-2-AP cible Alpha Actinin dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha Actinin Protéine recombinante Ag1859
• Nom complet: actinin, alpha 1
• Masse moléculaire calculée: 103 kDa
• Poids moléculaire observé: 100-105 kDa
• Numéro d’acquisition GenBank: BC015766
• Symbole du gène: Alpha Actinin
• Identification du gène (NCBI): 87
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

Publications

Species	Application	Title
mouse	IF	Redox Biol; MK-886 protects against cardiac ischaemia/reperfusion injury by activating proteasome-Keap1-NRF2 signalling; Authors - Kai-Na Shi
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
human	IF	Adv Healthc Mater; A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.; Authors - Zhi Zheng
mouse	WB	Cell Death Differ; Inhibition of cGAS-STING by JQ1 alleviates oxidative stress-induced retina inflammation and degeneration.; Authors - Ming Zou
mouse	IF	Transl Res; Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling.; Authors - Pengyu Jia
mouse	WB	Theranostics; miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.; Authors - Jing Shi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jason (Verified Customer) (10-28-2019)

Excellent antibody with consistent results at a 1/100 dilution on formalin fixed tissue.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Mouse myocardium

Alpha Actinin Antibody Immunohistochemistry, validation (1/100 dilution) in Mouse myocardium (Cat no:11313-2-AP)

FH

Steeve (Verified Customer) (01-08-2018)

Tested with another actinin mouse. Gave 1:1 ratio actinin:actinin

• Applications: Western Blot
• Primary Antibody Dilution: 1:1'1000
• Cell Tissue Type: human umbilical veins

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