Anticorps Polyclonal de lapin anti-ACTN2

ACTN2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

17

Avis (1)

N° de cat : 14221-1-AP

Synonymes

actinin, alpha 2, ACTN2, Alpha actinin 2, CMD1AA, F actin cross linking protein

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Galerie de données de validation

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  WB analysis using 14221-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 14221-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 14221-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 14221-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14221-1-AP (ACTN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14221-1-AP

  IP Result of anti-ACTN2 (IP:14221-1-AP, 4ug; Detection:14221-1-AP 1:1000) with HeLa cells lysate 1080ug.

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  IHC staining of mouse heart using 14221-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 14221-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 14221-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 14221-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14221-1-AP (ACTN2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Human iPSC derived cardiomyocyte using 14221-1-AP

  Human iPSC derived cardiomyocyte in 2D culture, with alpha actinin (14221-1-AP) and DAPI. Image courtesy of Chandan Kadur Nagaraju, Experimental Cardiology, KU Leuven, Belgium.

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  IF Staining of mouse heart using 14221-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 14221-1-AP (ACTN2 antibody) at dilution of 1:400 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 14221-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 14221-1-AP (ACTN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 14221-1-AP

  Mouse cardiac tissue slice of 300µm thickness labelled with alpha actinin (14221-1-AP). Image courtesy of Chandan Kadur Nagaraju, Experimental Cardiology, KU Leuven, Belgium.

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  IF Staining of C2C12 using 14221-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using 14221-1-AP (ACTN2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 14221-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using ACTN2 antibody (14221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules C2C12, cœur de souris, muscle squelettique de rat, rein de souris, tissu de muscle squelettique de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cardiaque de souris, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cardiaque de souris, Cardiomyocyte humain dérivé de CSPi, cellules C2C12, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Informations sur le produit

14221-1-AP cible ACTN2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACTN2 Protéine recombinante Ag5459
• Nom complet: actinin, alpha 2
• Masse moléculaire calculée: 104 kDa
• Poids moléculaire observé: 103 kDa
• Numéro d’acquisition GenBank: BC051770
• Symbole du gène: ACTN2
• Identification du gène (NCBI): 88
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).

Publications

Species	Application	Title
rat		Adv Mater; Exoskeleton Partial-Coated Stem Cells for Infarcted Myocardium Restoring; Authors - Huihui He
mouse	WB,IF	Nat Commun; Microskeletal stiffness promotes aortic aneurysm by sustaining pathological vascular smooth muscle cell mechanosensation via Piezo1.; Authors - Weiyi Qian
rat	WB	Basic Res Cardiol; Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.; Authors - Alzbeta Hulikova
human	IF	Dev Cell; Essential role of MESP1-RING1A complex in cardiac differentiation; Authors - Qianqian Liang
human	IF	Front Cell Dev Biol; Targeting of CAT and VCAM1 as Novel Therapeutic Targets for DMD Cardiomyopathy.; Authors - Bin Li
human	WB,IF	Front Cell Dev Biol; Incomplete Assembly of the Dystrophin-Associated Protein Complex in 2D and 3D-Cultured Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.; Authors - Guillaume Gilbert

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Rebecca (Verified Customer) (09-21-2022)

20ug of protein was loaded. Transference at 4ºC and 90V, for 2h. Antibody incubation ON at 4ºC.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: iPSC-CM

ACTN2 Antibody Western Blot validation (1:10000 dilution) in iPSC-CM (Cat no:14221-1-AP)