Anticorps Polyclonal de lapin anti-ARP2

• Phare
• Validé par KD/KO

ARP2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 10922-1-AP

Synonymes

Actin like protein 2, Actin related protein 2, ACTR2, ARP2

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Galerie de données de validation

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  WB analysis using 10922-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10922-1-AP (ARP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10922-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10922-1-AP (ARP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10922-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10922-1-AP (ARP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 10922-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10922-1-AP (ARP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 10922-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human ARP2 (10922-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules MCF-7, cellules SH-SY5Y, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293
• Résultats positifs en cytométrie: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IF: See 3 publications below

Informations sur le produit

10922-1-AP cible ARP2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARP2 Protéine recombinante Ag1359
• Nom complet: ARP2 actin-related protein 2 homolog (yeast)
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC014546
• Symbole du gène: ACTR2
• Identification du gène (NCBI): 10097
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.; Authors - Cao Jingli J ; KD Validated
human	WB	Nucleic Acids Res; G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities.; Authors - Xiaomei He
	WB	Elife; Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein; Authors - Congyan Zhang
human	WB	Elife; Capping protein regulates endosomal trafficking by controlling F-actin density around endocytic vesicles and recruiting RAB5 effectors.; Authors - Dawei Wang ; KD Validated
human	IF	Elife; mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex.; Authors - Madeline G Andrews
mouse	WB	Biol Reprod; RAB8A GTPase regulates spindle migration and Golgi apparatus distribution via ROCK-mediated actin assembly in mouse oocyte meiosis.; Authors - Zhen-Nan Pan