Anticorps Polyclonal de lapin anti-ARP3/ARP3B

• Phare
• Validé par KD/KO

ARP3/ARP3B Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 13822-1-AP

Synonymes

Actin like protein 3, Actin related protein 3, ACTR3, ACTR3B, Arp11, ARP3, ARP3/ARP3B, Arp4

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Galerie de données de validation

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  WB analysis using 13822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3/ARP3B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13822-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13822-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13822-1-AP (ARP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 13822-1-AP

  IP Result of anti-ARP3 (IP:13822-1-AP, 4ug; Detection:13822-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.

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  IHC staining of human kidney using 13822-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13822-1-AP (ARP3/ARP3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HeLa, cellules HL-60, cellules Jurkat, cellules K-562, cellules PC-3, tissu hépatique humain, tissu splénique de souris
• Résultats positifs en IP: tissu splénique de souris
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

13822-1-AP cible ARP3/ARP3B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARP3/ARP3B Protéine recombinante Ag4922
• Nom complet: ARP3 actin-related protein 3 homolog (yeast)
• Masse moléculaire calculée: 418 aa 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC044590
• Symbole du gène: ACTR3
• Identification du gène (NCBI): 10096
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARP3 (also known as ACTR3) is a major constituent of the ARP2/3 complex that is necessary for nucleating actin polymerization at filament branches. ARP3 localizes to the lamellipodia of stationary and locomoting fibroblasts. Overexpression of ARP3 has been reported in several types of cancer cells. This antibody recognizes endogenous ARP3 and may cross-react with ATCR3B.

Publications

Species	Application	Title
human	WB	Nature; Actin cables and comet tails organize mitochondrial networks in mitosis.; Authors - Andrew S Moore
rat	WB,IF	ACS Nano; Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.; Authors - Yiyuan Kang
mouse	IF	PLoS Genet; N-wasp is required for structural integrity of the blood-testis barrier.; Authors - Xiang Xiao
human	IP	EMBO Rep; WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis.; Authors - Kailiang Zhao
	WB	Elife; Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein; Authors - Congyan Zhang
human	WB	Int J Biol Sci; Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.; Authors - Xin Dong