Anticorps Polyclonal de lapin anti-ACVRL1

ACVRL1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 14745-1-AP

Synonymes

Activin receptor like kinase 1, Activin receptor-like kinase 1, ACVRLK1, ALK 1, ALK1

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Galerie de données de validation

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  WB analysis of human placenta using 14745-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14745-1-AP (ACVRL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14745-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14745-1-AP (ACVRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 14745-1-AP

  IP result of anti-ACVRL1 (IP:14745-1-AP, 4ug; Detection:14745-1-AP 1:300) with Jurkat cells lysate 2400ug.

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  IHC staining of human heart using 14745-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14745-1-AP (ACVRL1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lymphoma using 14745-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14745-1-AP (ACVRL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 14745-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14745-1-AP (ACVRL1 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Jurkat using 14745-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug ACVRL1 Polyclonal antibody (14745-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain,
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu cardiaque humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

14745-1-AP cible ACVRL1 dans les applications de WB, IHC, IF, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACVRL1 Protéine recombinante Ag6449
• Nom complet: activin A receptor type II-like 1
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC042637
• Symbole du gène: ACVRL1
• Identification du gène (NCBI): 94
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.

Publications

Species	Application	Title
mouse,human	WB	Mol Cell; OTULIN allies with LUBAC to govern angiogenesis by editing ALK1 linear polyubiquitin.; Authors - Yesheng Fu
human	WB	Nat Commun; Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils.; Authors - Yusuke Kimishima
human	WB	J Mol Cell Cardiol; BMP9 attenuates occurrence of venous malformation by maintaining endothelial quiescence and strengthening vessel walls via SMAD1/5/ID1/α-SMA pathway.; Authors - Yongyun Li
human	WB,IP	Cell Biol Int; CHRDL2 promotes osteosarcoma cell proliferation and metastasis through the BMP-9/ PI3K/ AKT pathway.; Authors - Houping Chen
human	IHC	J Thorac Dis; Pulmonary inflammatory myofibroblastic tumor versus IgG4-related inflammatory pseudotumor: differential diagnosis based on a case series.; Authors - Longfei Zhu
mouse	WB,IHC	Biosci Biotechnol Biochem; BMP9 functions as a negative regulator in the myogenic differentiation of primary mouse myoblasts; Authors - Xiya Lu

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