Anticorps Polyclonal de lapin anti-ADAM10

• Phare
• Validé par KD/KO

ADAM10 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (2)

N° de cat : 25900-1-AP

Synonymes

AD10, ADAM 10, ADAM10, CD156c, CDw156, HsT18717, kuz, Kuzbanian protein homolog, MADM

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Galerie de données de validation

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  WB analysis using 25900-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25900-1-AP (ADAM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 25900-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 25900-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 25900-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 25900-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25900-1-AP (ADAM10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

25900-1-AP cible ADAM10 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADAM10 Protéine recombinante Ag23078
• Nom complet: ADAM metallopeptidase domain 10
• Masse moléculaire calculée: 748 aa, 84 kDa
• Poids moléculaire observé: 60-70 kDa, 90 kDa
• Numéro d’acquisition GenBank: BC126253
• Symbole du gène: ADAM10
• Identification du gène (NCBI): 102
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).

Publications

Species	Application	Title
human	WB,IF	Cell Death Dis; Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.; Authors - Bo Wu
human,mouse	WB,IHC,IF	Pharmacol Res; IDO1 can impair NK cells function against non-small cell lung cancer by downregulation of NKG2D Ligand via ADAM10.; Authors - Xin Fang
human	WB	Cell Prolif; Increased SPON1 promotes pancreatic ductal adenocarcinoma progression by enhancing IL-6 trans-signalling.; Authors - Yanmiao Huo
human	WB	Front Cell Dev Biol; NLGN3 Upregulates Expression of ADAM10 to Promote the Cleavage of NLGN3 via Activating the LYN Pathway in Human Gliomas.; Authors - Ning-Ning Dang
human	WB	Front Cell Dev Biol; Epigenetic Regulation of a Disintegrin and Metalloproteinase (ADAM) Transcription in Colorectal Cancer Cells: Involvement of β-Catenin, BRG1, and KDM4.; Authors - Lina Sun
mouse	WB	J Alzheimers Dis; Periodontitis Deteriorates Cognitive Function and Impairs Neurons and Glia in a Mouse Model of Alzheimer's Disease.; Authors - Xueshen Qian

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xiaoyu (Verified Customer) (02-07-2024)

Good signal

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: brain

ADAM10 Antibody Western Blot validation (1:1000 dilution) in brain (Cat no:25900-1-AP)

FH

David (Verified Customer) (09-02-2019)

Used for immunoblot at 1:1000 which gives good, clear bands at the expected molecular weight, but does require long exposure times (30µg total protein, 10-15 minutes exposure). 1:500 would probably be a better dilution and this will be used for future experiments. It can be difficult to obtain good ADAM10 antibodies, but after trying several, this is the best.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human neuroblastoma