Anticorps Polyclonal de lapin anti-ADAM12

ADAM12 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

N° de cat : 26928-1-AP

Synonymes

ADAM 12, ADAM12, MCMP, MCMPMltna, Meltrin alpha, MLTN, MLTNA

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Galerie de données de validation

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  WB analysis using 26928-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 26928-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 26928-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26928-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26928-1-AP (ADAM12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 26928-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 26928-1-AP (ADAM12 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 26928-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 26928-1-AP (ADAM12 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A431, cellules A549, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

26928-1-AP cible ADAM12 dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADAM12 Protéine recombinante Ag17474
• Nom complet: ADAM metallopeptidase domain 12
• Masse moléculaire calculée: 909 aa, 100 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC060804
• Symbole du gène: ADAM12
• Identification du gène (NCBI): 8038
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors. It is involved in the regulation of growth factor activities and integrin functions, leading to promotion of cell growth and invasion, although the precise mechanisms of these are not clear at the present time.(PMID:17355265) ADAM12's ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein's role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kd full-length form, 90 kDa mature (processed form that lack the prodomain), 50-68 kDa degradation product and 27 kDa prodomain.