Anticorps Recombinant de lapin anti-ADAM17

ADAM17 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241305A2

N° de cat : 84292-4-RR

Synonymes

241305A2, ADAM 17, cSVP, Disintegrin and metalloproteinase domain-containing protein 17, EC:3.4.24.86

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Galerie de données de validation

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  WB analysis using 84292-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84292-4-RR (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human rectal cancer using 84292-4-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84292-4-RR (ADAM17 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HCT 116 using 84292-4-RR

  1x10^6 HCT 116 cells were intracellularly stained with 0.25 ug ADAM17 Recombinant antibody (84292-4-RR, Clone:241305A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84292-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84292-4-RR against Human ADAM17 were performed. The affinity constant is 0.385 nM.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HCT 116, cellules HeLa, cellules HUVEC, cellules RAW 264.7, cellules SW480
• Résultats positifs en IHC: human rectal cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HCT 116,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84292-4-RR cible ADAM17 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ADAM17 Protéine recombinante Ag32418
• Nom complet: ADAM metallopeptidase domain 17
• Masse moléculaire calculée: 824 aa, 93 kDa
• Poids moléculaire observé: 100-130 kDa
• Numéro d’acquisition GenBank: BC136783
• Symbole du gène: ADAM17
• Identification du gène (NCBI): 6868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).

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