Anticorps Polyclonal de lapin anti-ADAM28

• Phare
• Validé par KD/KO

ADAM28 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 22234-1-AP

Synonymes

ADAM 28, ADAM28, eMDC II, eMDCII, MDC L, MDC Lm, MDC Ls, MDCL

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Galerie de données de validation

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  WB analysis of mouse lung using 22234-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 22234-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 22234-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22234-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 22234-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 22234-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 22234-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 22234-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 22234-1-AP (ADAM28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 22234-1-AP

  IP Result of anti-ADAM28 (IP:22234-1-AP, 4ug; Detection:22234-1-AP 1:800) with mouse lung tissue lysate 3600ug.

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules BxPC-3, cellules PC-13, tissu de côlon de souris, tissu d'intestin grêle de souris, tissu hépatique de souris, tissu splénique de souris
• Résultats positifs en IP: tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below

Informations sur le produit

22234-1-AP cible ADAM28 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADAM28 Protéine recombinante Ag17586
• Nom complet: ADAM metallopeptidase domain 28
• Masse moléculaire calculée: 775 aa, 87 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC136478
• Symbole du gène: ADAM28
• Identification du gène (NCBI): 10863
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADAM28 belongs to a family of cell surface and secreted glycoproteins that possess both proteolytic and adhesive properties. ADAM28 is predominantly expressed by carcinoma cells in the breast carcinoma tissues as protein bands of 42 kDa and 55/57 kDa, which correspond to the active forms of ADAM28s and ADAM28m, respectively.In human non-small cell lung carcinomas and breast carcinomas, ADAM28 is overexpressed predominantly by carcinoma cells, and the expression correlates with carcinoma cell proliferation and lymph node metastasis(PMID:19601836).

Publications

Species	Application	Title
mouse	WB	Nat Commun; A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility; Authors - Daiji Kiyozumi
human	WB	Cancer Lett; ADAM28 promotes tumor growth and dissemination of acute myeloid leukemia through IGFBP-3 degradation and IGF-I-induced cell proliferation.; Authors - Jia-Min Zhang ; KO Validated
mouse	WB	Biol Reprod; ADGRG2 is dispensable for lumicrine signalling regulating epididymal initial segment differentiation and gene expression†; Authors - Daiji Kiyozumi
human	WB	World J Gastroenterol; PMID: 31602160; Authors - Li Wei ; KD Validated
mouse	WB	Mol Cell Neurosci; Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR.; Authors - Zhen-Huang Chen