Anticorps Polyclonal de lapin anti-ADAM8

• Phare
• Validé par KD/KO

ADAM8 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 23778-1-AP

Synonymes

ADAM metallopeptidase domain 8, ADAM8, ADAM8 protein, CD156, MS2

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Galerie de données de validation

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  WB analysis of BxPC-3 using 23778-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 23778-1-AP (ADAM8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 23778-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 23778-1-AP (ADAM8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 23778-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23778-1-AP (ADAM8 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 23778-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23778-1-AP (ADAM8 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 23778-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23778-1-AP (ADAM8 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 23778-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23778-1-AP (ADAM8 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, tissu pancréatique de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

23778-1-AP cible ADAM8 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADAM8 Protéine recombinante Ag17262
• Nom complet: ADAM metallopeptidase domain 8
• Masse moléculaire calculée: 824 aa, 89 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC115404
• Symbole du gène: ADAM8
• Identification du gène (NCBI): 101
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ADAMs are a large family of transmembrane proteins that are involved in proteolysis, making them candidates for mediating the remodeling of the extracellular matrix(ECM). ADAM8, one of the members of the ADAM family, is overexpressed in various human tumors. It has been shown previously that hypoxia influences the expression of some ADAM proteins. The protein found to be expressed in two active forms(90 kDa and 60 kDa): potential shedding protein and remnant protein in the pancreatic tissues under chronic pancreatitis and pancreatic cancer(PMID:18566576). The full length protein has a signal peptide and four glycosylation sites. This antibody is specific to ADAM8.

Publications

Species	Application	Title
mouse	IHC	Mol Cell Neurosci; Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR.; Authors - Zhen-Huang Chen
mouse	WB,IHC	Exp Anim; Suppression of ADAM8 attenuates angiotensin II-induced cardiac fibrosis and endothelial-mesenchymal transition via inhibiting TGF-β1/Smad2/Smad3 pathways.; Authors - Lixia Yao ; KD Validated
mouse	WB,IP	J Healthc Eng; ADAM8 Activates NLRP3 Inflammasome to Promote Cerebral Ischemia-Reperfusion Injury.; Authors - Hongwei Lu
human	WB	Pathol Oncol Res; High ADAM8 expression is associated with poor prognosis in patients with hepatocellular carcinoma.; Authors - Yun Zhang
human	WB,IHC	Int J Mol Med; ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.; Authors - Wei Li
mouse	WB	Nat Med; Translation control of the immune checkpoint in cancer and its therapeutic targeting.; Authors - Yichen Xu