Anticorps Polyclonal de lapin anti-ADH1B

ADH1B Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 17165-1-AP

Synonymes

ADH1B, ADH2, Alcohol dehydrogenase 1B

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Galerie de données de validation

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  WB analysis of mouse liver using 17165-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17165-1-AP (ADH1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 17165-1-AP

  IP result of anti-ADH1B (IP:17165-1-AP, 4ug; Detection:17165-1-AP 1:500) with mouse liver tissue lysate 4000 ug.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

17165-1-AP cible ADH1B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ADH1B Protéine recombinante Ag10630
• Nom complet: alcohol dehydrogenase 1B (class I), beta polypeptide
• Masse moléculaire calculée: 375 aa, 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC033009
• Symbole du gène: ADH1B
• Identification du gène (NCBI): 125
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).

Publications

Species	Application	Title
	IF	Cell Death Discov; Senescence as a dictator of patient outcomes and therapeutic efficacies in human gastric cancer.; Authors - Lulin Zhou
	IF	Endocrinology; De novo-synthesized retinoic acid in ovarian antral follicles enhances FSH-mediated ovarian follicular cell differentiation and female fertility.; Authors - Tomoko Kawai
rat	WB	Arch Biochem Biophys; Baicalin ameliorates alcohol-induced hepatic steatosis by suppressing SREBP1c elicited PNPLA3 competitive binding to ATGL.; Authors - Pan Li
	IHC	Exp Eye Res; Proteomic analysis reveals the heterogeneity of metabolic reprogramming in lacrimal gland tumors.; Authors - Jie Yang
rat	WB	J Sci Food Agric; Probiotic-fermented Pueraria lobata (Willd.) Ohwi alleviates alcoholic liver injury by enhancing antioxidant defense and modulating gut microbiota; Authors - Wenjun Zhao
	WB	Front Pharmacol; Identification of afatinib-associated ADH1B and potential small-molecule drugs targeting ADH1B for hepatocellular carcinoma; Authors - Yongxu Zhou